Detailed information of TA system TA138398

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 91207..91633
• Replicon: plasmid p1_115106
• Accession: NZ_CP043336
• Organism: Escherichia coli strain WCHEC115106

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: FZC46_RS24150
• Protein ID: WP_001372321.1
• Coordinates: 91207..91332 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	91409..91633 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZC46_RS24110 (86576)	86576..87265	-	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-
FZC46_RS24115 (87452)	87452..87835	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
FZC46_RS24120 (88156)	88156..88758	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
FZC46_RS24125 (89055)	89055..89876	-	822	WP_072795862.1	DUF932 domain-containing protein	-
FZC46_RS24130 (89998)	89998..90285	-	288	WP_000107535.1	hypothetical protein	-
FZC46_RS24135 (90310)	90310..90516	-	207	WP_000547968.1	hypothetical protein	-
FZC46_RS24140 (90586)	90586..90759	+	174	Protein_104	hypothetical protein	-
FZC46_RS24145 (90757)	90757..90987	-	231	WP_001426396.1	hypothetical protein	-
FZC46_RS24150 (91207)	91207..91332	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
FZC46_RS24155 (91274)	91274..91423	-	150	Protein_107	plasmid maintenance protein Mok	-
- (91409)	91409..91633	-	225	NuclAT_0	-	Antitoxin
- (91409)	91409..91633	-	225	NuclAT_0	-	Antitoxin
- (91409)	91409..91633	-	225	NuclAT_0	-	Antitoxin
- (91409)	91409..91633	-	225	NuclAT_0	-	Antitoxin
FZC46_RS24160 (91445)	91445..91633	+	189	WP_001299721.1	hypothetical protein	-
FZC46_RS24165 (91602)	91602..92364	-	763	Protein_109	plasmid SOS inhibition protein A	-
FZC46_RS24170 (92361)	92361..92795	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FZC46_RS24175 (92850)	92850..94808	-	1959	WP_241420247.1	ParB/RepB/Spo0J family partition protein	-
FZC46_RS24180 (94873)	94873..95106	-	234	WP_000005987.1	DUF905 family protein	-
FZC46_RS24185 (95163)	95163..95702	-	540	WP_032332913.1	single-stranded DNA-binding protein	-
FZC46_RS24190 (95728)	95728..95934	-	207	WP_000275853.1	hypothetical protein	-
FZC46_RS24195 (96175)	96175..96402	-	228	WP_071961421.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / lnu(F) / blaTEM-1B / sul3 / aph(3')-Ia / aac(3)-IId	-	1..133446	133446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T138398 WP_001372321.1 NZ_CP043336:c91332-91207 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T138398 NZ_CP057681:3776925-3777077 [Escherichia fergusonii]
ATGCTGACAAAATATGCCCTTGTGGCAATCATCGTACTGTGTTGTACAGTACTGGGATTCACGCTGATGGTAGGCGACTC
GTTGTGTGAGTTGAGTATCAGAGAACGTGGTATGGAGTTTAAGGCAGTTCTCGCTTACGAATCGAAGAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT138398 NZ_CP043336:c91633-91409 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin