Detailed information of TA system TA138363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5914492..5915087
• Replicon: chromosome
• Accession: NZ_CP043328
• Organism: Pseudomonas aeruginosa strain CCUG 51971

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: FZE14_RS28625
• Protein ID: WP_003113526.1
• Coordinates: 5914809..5915087 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FZE14_RS28620
• Protein ID: WP_003133769.1
• Coordinates: 5914492..5914797 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZE14_RS28585	5909643..5910491	+	849	WP_003095007.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
FZE14_RS28595	5910658..5911599	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
FZE14_RS28600	5911716..5912330	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
FZE14_RS28605	5912372..5912956	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
FZE14_RS28610	5912997..5914097	+	1101	WP_003095017.1	redox-regulated ATPase YchF	-
FZE14_RS28620	5914492..5914797	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
FZE14_RS28625	5914809..5915087	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FZE14_RS28635	5915416..5917644	+	2229	WP_023104079.1	TonB-dependent receptor	-
FZE14_RS28640	5917715..5918362	-	648	WP_003095021.1	carbonate dehydratase	-
FZE14_RS28645	5918424..5919662	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T138363 WP_003113526.1 NZ_CP043328:c5915087-5914809 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T138363 NZ_CP057632:c4010938-4010531 [Citrobacter sp. RHB20-C15]
GTGGTCCTGTGGCAATCTGATCTCCGCGTCTCATGGCGCGCACAGTGGCTATCACTCCTTCTGCATGGGCTGGTGGCCGC
GGCGATTCTATTAATGCCATGGCCGCTGAGCTACACGCCGCTGTGGTTGATACTGCTTTCGCTGGTGGTATTTGACTGTG
TCCGCAGCCAGCGGCGAATTAACGCCCGTCAGGGAGAGGTCAAACTTCTGATGGATGGACGACTCCGCTGGCAGGGGCAG
GACTGGCTTATCGTCAGTGCACCGTGGATGATCAAAACCGGCATGATGCTGCGCTTGCGCTCAGAAACCGGTAAACGCCA
GCATTTGTGGCTCGCGGCTGACAGTATGGATAAGGCGGAATGGCGGGATTTACGCCGCCTGATGATGCAACCGTCGACGC
AAGGGTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT138363 WP_003133769.1 NZ_CP043328:c5914797-5914492 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT138363 NZ_CP057632:c4011185-4010919 [Citrobacter sp. RHB20-C15]
ATGGATATTAACAATAAAGCACGTATTCACTGGGCATGCCGACGCGGTATGCGTGAACTCGATATTTCCATCATGCCGTT
TTTCGAACATGAATACGACACGTTGAGTGATGATGAAAAACGTATTTTTATCCGCCTGCTGGAGTGTGATGACCCGGATT
TATTCAACTGGTTGATGAATCACGGTAAACCGGCAGATGCTGAACTGGAGCAGATGGTACGACTCATCCAGACACGGAAT
CGGGATCGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V6ALY3