Detailed information of TA system TA138354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1740866..1741440
• Replicon: chromosome
• Accession: NZ_CP043320
• Organism: Pseudomonas sp. C27(2019)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FXF61_RS07895
• Protein ID: WP_151184756.1
• Coordinates: 1741162..1741440 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FXF61_RS07890
• Protein ID: WP_151184755.1
• Coordinates: 1740866..1741162 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXF61_RS07880	1736447..1739704	-	3258	WP_151184753.1	type I restriction endonuclease subunit R	-
FXF61_RS07885	1739748..1740638	-	891	WP_178087339.1	virulence RhuM family protein	-
FXF61_RS07890	1740866..1741162	-	297	WP_151184755.1	HigA family addiction module antidote protein	Antitoxin
FXF61_RS07895	1741162..1741440	-	279	WP_151184756.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FXF61_RS07900	1741827..1742051	-	225	WP_151184757.1	addiction module protein	-
FXF61_RS07905	1742201..1743313	-	1113	WP_151184758.1	Fic family protein	-
FXF61_RS07910	1743410..1745245	-	1836	WP_151184759.1	DUF262 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1733867..1746706	12839

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10784.25 Da
• Isoelectric Point: 8.4954

>T138354 WP_151184756.1 NZ_CP043320:c1741440-1741162 [Pseudomonas sp. C27(2019)]
MIKNFIHKGLQRFYSSGNTSGIQTKHEKKLRLILTNLEQAATPDDMDLPGLFMHQLKGDRKDIWSVRVSGNWRVTYRFVG
DDVEIVNYEDYH

Nucleotide

• Length: 279 bp

>T138354 NZ_CP057630:c4696825-4696439 [Citrobacter sp. RHB20-C16]
ATGCAAAAGGGACCCGTGTTGTTCGATACCAATATCCTGATTGATTTATTTAGCGGGCGCCAGGAAGCACAGCAAGTACT
GGAAGCGTATCCGCCGCAGAATGCCATCAGCTTGATTACCTGGATGGAAGTCATGGTGGGGGCAAAAAAATACCATCAGG
AATATCGTACGCGTGTGGCATTGAGTGCGTTCAATATCATCGGTGTATCACTGGAAATTGCTGAGCGAAGCGTGAATCTC
AGGCAGGAATATGGGATGAAACTTCCGGACGCGATCATTCTGGCTACCGCGCAGATTCATCGGTTTGCGCTTGTGACGCG
CAACACCAGAGATTTCGCGGGGATACCTGGCGTCATTACGCCTTATCAGTTGCAGGCCGGACGATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11063.82 Da
• Isoelectric Point: 8.6274

>AT138354 WP_151184755.1 NZ_CP043320:c1741162-1740866 [Pseudomonas sp. C27(2019)]
MTMFNPAHPGEILKELIIDALGLTITDAANHLNISRKTLSKVLNARGAITPEMAVRLELVFGKPSADHWLRLQSAYDLWQ
MRQHQDQLNVSPYKIQTA

Nucleotide

• Length: 297 bp

>AT138354 NZ_CP043320:c1741162-1740866 [Pseudomonas sp. C27(2019)]
ATGACTATGTTTAACCCAGCACACCCCGGTGAAATCCTGAAAGAACTCATCATCGATGCCTTGGGCCTGACCATTACTGA
TGCGGCCAACCATTTAAATATCAGCCGTAAAACACTGTCTAAAGTTCTGAACGCACGCGGTGCTATCACGCCTGAAATGG
CTGTGCGTTTAGAACTGGTGTTTGGCAAACCCTCAGCAGATCATTGGCTGCGCCTGCAAAGTGCCTATGATCTTTGGCAA
ATGAGGCAGCATCAAGACCAGTTAAATGTCTCACCATATAAGATACAAACTGCATAA