Detailed information of TA system TA138322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 50577..51162
• Replicon: plasmid pAcsw19-2
• Accession: NZ_CP043309
• Organism: Acinetobacter johnsonii strain Acsw19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: FYN22_RS16780
• Protein ID: WP_000897307.1
• Coordinates: 50842..51162 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FYN22_RS16775
• Protein ID: WP_000369781.1
• Coordinates: 50577..50849 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYN22_RS16755	45650..46612	-	963	WP_033917576.1	IS30 family transposase	-
FYN22_RS16760	46868..47479	+	612	WP_015060246.1	recombinase family protein	-
FYN22_RS16765	47669..48553	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
FYN22_RS16770	48609..50084	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
FYN22_RS16775	50577..50849	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
FYN22_RS16780	50842..51162	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FYN22_RS16785	51302..51721	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
FYN22_RS16790	51801..52082	-	282	WP_000985609.1	putative addiction module antidote protein	-
FYN22_RS16795	52083..52385	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FYN22_RS16800	52534..53064	+	531	WP_000172759.1	hypothetical protein	-
FYN22_RS16805	53153..53467	-	315	WP_004728120.1	BrnA antitoxin family protein	-
FYN22_RS16810	53454..53741	-	288	WP_000438825.1	BrnT family toxin	-
FYN22_RS16815	53971..54576	-	606	WP_000447788.1	LysE family translocator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	cmlB1 / mph(E) / msr(E) / blaOXA-58 / blaNDM-1 / aph(3')-VIa	-	1..351885	351885
	-	inside	IScluster/Tn	mph(E) / msr(E) / blaOXA-58	-	45650..62451	16801

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T138322 WP_000897307.1 NZ_CP043309:c51162-50842 [Acinetobacter johnsonii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T138322 NZ_CP057565:3288152-3288370 [Escherichia fergusonii]
ATGTCCGATAAGCCTTTAACCAAAACTGATTATTTGATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAACGTGT
CATCGAAAAAAATAAATACGAACTCTCTGACAATGAACTGGCGGTATTTTATTCAGCAGCTGATCACCGTCTCGCTGAAT
TGACCATGAATAAACTTTACGACAAGATCCCCTCCTCCGTATGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT138322 WP_000369781.1 NZ_CP043309:c50849-50577 [Acinetobacter johnsonii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT138322 NZ_CP057565:3287751-3288125 [Escherichia fergusonii]
ATGGACGAATACTCACCAAAACGACATGATATCGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAATCTTGAAGAGAGCAACCATGGCTGGGTTAACGACCCAACCTCGGCTATCAATCTGCAGCTCAACGAACTGATTGAGC
ATATTGCAACCTTCGCACTTAATTACAAAATTAAGTATAATGAGGACAATAAGCTCATAGAGCAGATCGATGAATATCTG
GATGACACTTTTATGTTGTTCAGTAGTTATGGCATTAATACACAGGATTTACAGAAATGGCGAAAATCTGGAAACAGGCT
CTTCCGCTGTTTTGTTAACGCCACCAGGGCGAACCCTGTTAGTTTGTCGTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3