Detailed information of TA system TA138298

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85293..85719
• Replicon: plasmid p1_040047
• Accession: NZ_CP043297
• Organism: Escherichia coli strain WCHEC040047

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: FYK54_RS25355
• Protein ID: WP_001372321.1
• Coordinates: 85293..85418 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	85495..85719 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK54_RS25315 (80667)	80667..81356	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
FYK54_RS25320 (81543)	81543..81926	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
FYK54_RS25325 (82247)	82247..82849	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
FYK54_RS25330 (83146)	83146..83967	-	822	WP_001234426.1	DUF932 domain-containing protein	-
FYK54_RS25335 (84085)	84085..84372	-	288	WP_000107535.1	hypothetical protein	-
FYK54_RS25340 (84397)	84397..84603	-	207	WP_000547968.1	hypothetical protein	-
FYK54_RS25345 (84673)	84673..84845	+	173	Protein_106	hypothetical protein	-
FYK54_RS25350 (84843)	84843..85073	-	231	WP_071586998.1	hypothetical protein	-
FYK54_RS25355 (85293)	85293..85418	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
FYK54_RS25360 (85360)	85360..85509	-	150	Protein_109	plasmid maintenance protein Mok	-
- (85495)	85495..85719	-	225	NuclAT_0	-	Antitoxin
- (85495)	85495..85719	-	225	NuclAT_0	-	Antitoxin
- (85495)	85495..85719	-	225	NuclAT_0	-	Antitoxin
- (85495)	85495..85719	-	225	NuclAT_0	-	Antitoxin
FYK54_RS25365 (85531)	85531..85719	+	189	WP_001299721.1	hypothetical protein	-
FYK54_RS25370 (85688)	85688..86450	-	763	Protein_111	plasmid SOS inhibition protein A	-
FYK54_RS25375 (86447)	86447..86881	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
FYK54_RS25380 (86936)	86936..87133	-	198	Protein_113	hypothetical protein	-
FYK54_RS25385 (87161)	87161..87394	-	234	WP_000005987.1	DUF905 family protein	-
FYK54_RS25390 (87462)	87462..88001	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
FYK54_RS25395 (88027)	88027..88233	-	207	WP_000275856.1	hypothetical protein	-
FYK54_RS25400 (88303)	88303..88383	+	81	Protein_117	hypothetical protein	-
FYK54_RS25405 (88566)	88566..88735	-	170	Protein_118	hypothetical protein	-
FYK54_RS25410 (89372)	89372..90343	-	972	WP_112917248.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIa / sitABCD	iutA / iucD / iucC / iucB / iucA	1..110724	110724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T138298 WP_001372321.1 NZ_CP043297:c85418-85293 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T138298 NZ_CP057509:4374263-4374577 [Citrobacter freundii]
ATGGAAATTCTTCAGACAATGGTTTTCCAGCGTTGGGAGCAAAACCTACGGGACAGGAGAGCTAAAACGCTCATTGCTAC
ACGCCTTTTTCGTTTGGCTAATGGTTTAGCTGGTGATATCAAACCTGTTGGAGAAGGCATCAGCGAAATGCGCATTTCCT
ACGGTCCTGGTTACAGGATTTACTTTAAACAGCAAGGCTGCCACATCATCATTTTGTTATGTGGGGGCGATAAAAGCTCA
CAAGCCAACGATATCACTATGGCAAAAATGCTCGCCCGTTCACTGGATTATCAAGAGATCCGCCACCATGAATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT138298 NZ_CP043297:c85719-85495 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin