Detailed information of TA system TA138293

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44257..44511
• Replicon: plasmid p1_040047
• Accession: NZ_CP043297
• Organism: Escherichia coli strain WCHEC040047

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: FYK54_RS25100
• Protein ID: WP_001312851.1
• Coordinates: 44257..44406 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	44450..44511 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK54_RS25060 (39809)	39809..40210	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
FYK54_RS25065 (40143)	40143..40400	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
FYK54_RS25070 (40493)	40493..41146	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
FYK54_RS25075 (42085)	42085..42942	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
FYK54_RS25080 (42935)	42935..43417	-	483	WP_001273588.1	hypothetical protein	-
FYK54_RS25085 (43410)	43410..43457	-	48	WP_229471593.1	hypothetical protein	-
FYK54_RS25090 (43448)	43448..43699	+	252	WP_223195197.1	replication protein RepA	-
FYK54_RS25095 (43716)	43716..43973	-	258	WP_000083845.1	replication regulatory protein RepA	-
FYK54_RS25100 (44257)	44257..44406	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (44450)	44450..44511	+	62	NuclAT_1	-	Antitoxin
- (44450)	44450..44511	+	62	NuclAT_1	-	Antitoxin
- (44450)	44450..44511	+	62	NuclAT_1	-	Antitoxin
- (44450)	44450..44511	+	62	NuclAT_1	-	Antitoxin
FYK54_RS25105 (44767)	44767..44841	-	75	Protein_58	endonuclease	-
FYK54_RS25110 (45087)	45087..45299	-	213	WP_001299730.1	ANR family transcriptional regulator	-
FYK54_RS25115 (45435)	45435..45995	-	561	WP_112917249.1	fertility inhibition protein FinO	-
FYK54_RS25120 (46098)	46098..46958	-	861	WP_000704513.1	alpha/beta hydrolase	-
FYK54_RS25125 (47017)	47017..47763	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIa / sitABCD	iutA / iucD / iucC / iucB / iucA	1..110724	110724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T138293 WP_001312851.1 NZ_CP043297:c44406-44257 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T138293 NZ_CP057509:c2314706-2314224 [Citrobacter freundii]
ATGGAAATAAACGTCACGGCGCCGGCGTTGCTGACCGAAGGGCATGAGCTGCAGCCATTCGATTGCGGTAATGAGGTGTT
AAATGATTGGCTACGTCGCCGGGCGATTAAAAATCAGTACCTCAACGCATCGCGCACATTCGTCATTTGTCTGGAGTCTA
CTCAGCGCGTAGTGGGTTACTATTCCATTGCAACTGGATCCGTGAGCCACGCTGACTTAGGGCGTAGTTTACGTCAGAAT
ATGCCCGATCCTGTTCCGGTTGTTCTGTTGGGCCGTCTTGCTGTTGATGCCAGTACGCAAGGTCATAGTTTTGGAAAATG
GCTGCTTAATGATGCTGTACTGCGCATTTCCAACTTAGCCGATCAAGTGGGTATCAAAGCCATCATGGTACATGCTATTG
ATGGCAAGGCCAGAGCGTTCTATGAATATTTCGGTTTCGTTCAGTCCCCAATAGCGGCAAATACGTTGTTCTACAAAATT
TAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT138293 NZ_CP043297:44450-44511 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin