Detailed information of TA system TA138281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3787459..3788186
• Replicon: chromosome
• Accession: NZ_CP043295
• Organism: Escherichia coli strain WCHEC040047

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: FYK54_RS18135
• Protein ID: WP_001521139.1
• Coordinates: 3787459..3787770 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FYK54_RS18140
• Protein ID: WP_000126294.1
• Coordinates: 3787767..3788186 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK54_RS18110 (3783394)	3783394..3785103	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
FYK54_RS18115 (3785113)	3785113..3785655	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
FYK54_RS18120 (3785655)	3785655..3786422	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
FYK54_RS18125 (3786419)	3786419..3786829	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
FYK54_RS18130 (3786822)	3786822..3787292	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
FYK54_RS18135 (3787459)	3787459..3787770	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
FYK54_RS18140 (3787767)	3787767..3788186	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
FYK54_RS18145 (3788265)	3788265..3789689	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
FYK54_RS18150 (3789698)	3789698..3791155	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FYK54_RS18155 (3791415)	3791415..3792425	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
FYK54_RS18160 (3792574)	3792574..3793101	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T138281 WP_001521139.1 NZ_CP043295:3787459-3787770 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T138281 NZ_CP057506:c1209937-1209596 [Citrobacter freundii]
ATGAAACCTCAATCTGCAGCAACTTCGCGGGCGGTAAAACCCTGCCTGACACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGCCTGCTGGAACAACACTATGGTCTTACCCTGAACGATACTCCTTTTATTGATGAAACCGTTATTCAGAATCACATTA
ATGCCGGGATCACGCTTGCTGATGCCGTTAACTTTCTGGTGGATAAGTACGAGCTTGTTCGCATCGATCGCAGAAGGTTT
AGTTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAA
CCGTAACAATGCAGTACGCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT138281 WP_000126294.1 NZ_CP043295:3787767-3788186 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT138281 NZ_CP057506:c1210275-1209958 [Citrobacter freundii]
ATGAGCAATATCACATGGGGCCTGCAGCGGGATATCACACCGCGCCTGGGGGCCCGTCTGGTACAGGAAAGAAACCGGCT
GCATTATCTGGCTGACCGTGCCAGCATCACCGGTAAATTCAGTGACGCCGAATGCCAGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCACCGGTGAAATGAATCCTCGCCATGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATATATCGCCGTTTACCCCACTAAGCGCTAA

Structures

Toxin

• AlphaFold DB: A0A829L2T9