Detailed information of TA system TA138277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3366865..3367558
• Replicon: chromosome
• Accession: NZ_CP043295
• Organism: Escherichia coli strain WCHEC040047

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: FYK54_RS16150
• Protein ID: WP_000415584.1
• Coordinates: 3366865..3367161 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: FYK54_RS16155
• Protein ID: WP_000650107.1
• Coordinates: 3367163..3367558 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK54_RS16115 (3361953)	3361953..3362267	-	315	WP_000958598.1	putative quinol monooxygenase	-
FYK54_RS16120 (3362298)	3362298..3362879	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
FYK54_RS16125 (3363198)	3363198..3363530	+	333	WP_023149630.1	DUF2645 family protein	-
FYK54_RS16130 (3363576)	3363576..3364925	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
FYK54_RS16135 (3364922)	3364922..3365581	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
FYK54_RS16140 (3365733)	3365733..3366125	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
FYK54_RS16145 (3366178)	3366178..3366660	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
FYK54_RS16150 (3366865)	3366865..3367161	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
FYK54_RS16155 (3367163)	3367163..3367558	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
FYK54_RS16160 (3367691)	3367691..3369298	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
FYK54_RS16165 (3369436)	3369436..3371694	+	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T138277 WP_000415584.1 NZ_CP043295:3366865-3367161 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T138277 NZ_CP043295:3366865-3367161 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT138277 WP_000650107.1 NZ_CP043295:3367163-3367558 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT138277 NZ_CP043295:3367163-3367558 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65