Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1527236..1527930 Replicon chromosome
Accession NZ_CP043295
Organism Escherichia coli strain WCHEC040047

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag FYK54_RS07430 Protein ID WP_001521903.1
Coordinates 1527236..1527634 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FYK54_RS07435 Protein ID WP_000554758.1
Coordinates 1527637..1527930 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (1523066) 1523066..1523146 - 81 NuclAT_11 - -
- (1523066) 1523066..1523146 - 81 NuclAT_11 - -
- (1523066) 1523066..1523146 - 81 NuclAT_11 - -
- (1523066) 1523066..1523146 - 81 NuclAT_11 - -
FYK54_RS07400 (1522406) 1522406..1523650 - 1245 WP_000189541.1 esterase FrsA -
FYK54_RS07405 (1523742) 1523742..1524200 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
FYK54_RS07410 (1524461) 1524461..1525918 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
FYK54_RS07415 (1525975) 1525975..1526327 - 353 Protein_1455 peptide chain release factor H -
FYK54_RS07420 (1526323) 1526323..1526529 - 207 Protein_1456 RtcB family protein -
FYK54_RS07425 (1526774) 1526774..1527226 - 453 WP_023144376.1 GNAT family N-acetyltransferase -
FYK54_RS07430 (1527236) 1527236..1527634 - 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FYK54_RS07435 (1527637) 1527637..1527930 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FYK54_RS07440 (1527982) 1527982..1529037 - 1056 WP_001226177.1 DNA polymerase IV -
FYK54_RS07445 (1529108) 1529108..1529893 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
FYK54_RS07450 (1529865) 1529865..1531577 + 1713 Protein_1462 flagellar biosynthesis protein FlhA -
FYK54_RS07455 (1531656) 1531656..1532009 + 354 WP_024166466.1 type II toxin-antitoxin system HicB family antitoxin -
FYK54_RS07460 (1532066) 1532066..1532815 - 750 WP_016233951.1 C40 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1517173..1527930 10757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T138270 WP_001521903.1 NZ_CP043295:c1527634-1527236 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T138270 NZ_CP057505:c679791-679582 [Escherichia fergusonii]
ATGCTGAACACATGTAGAGTGCCTCTTACTGACCGTAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT138270 WP_000554758.1 NZ_CP043295:c1527930-1527637 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT138270 NZ_CP057505:679783-679840 [Escherichia fergusonii]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References