Detailed information of TA system TA138259

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27241..27510
• Replicon: plasmid p2_025985
• Accession: NZ_CP043289
• Organism: Escherichia coli strain WCHEC025985

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: FYK42_RS25670
• Protein ID: WP_001372321.1
• Coordinates: 27385..27510 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27241..27306 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK42_RS25630	22241..22708	-	468	Protein_29	hypothetical protein	-
FYK42_RS25635	23010..23537	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
FYK42_RS25640	23595..23828	+	234	WP_000006003.1	DUF905 family protein	-
FYK42_RS25645	23889..25853	+	1965	WP_117389470.1	ParB/RepB/Spo0J family partition protein	-
FYK42_RS25650	25922..26356	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FYK42_RS25655	26353..27115	+	763	Protein_34	plasmid SOS inhibition protein A	-
-	27084..27308	+	225	NuclAT_0	-	-
-	27084..27308	+	225	NuclAT_0	-	-
-	27084..27308	+	225	NuclAT_0	-	-
-	27084..27308	+	225	NuclAT_0	-	-
FYK42_RS25660	27093..27272	-	180	WP_001309233.1	hypothetical protein	-
-	27241..27306	+	66	-	-	Antitoxin
FYK42_RS25665	27294..27443	+	150	Protein_36	plasmid maintenance protein Mok	-
FYK42_RS25670	27385..27510	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
FYK42_RS25675	27811..28107	-	297	Protein_38	hypothetical protein	-
FYK42_RS25680	28407..28703	+	297	WP_001272251.1	hypothetical protein	-
FYK42_RS25685	28814..29635	+	822	WP_001234445.1	DUF932 domain-containing protein	-
FYK42_RS25690	29932..30522	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
FYK42_RS25695	30855..31238	+	384	WP_001151529.1	conjugal transfer relaxosome DNA-binding protein TraM	-
FYK42_RS25700	31430..32077	+	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
FYK42_RS25705	32197..32424	+	228	WP_000589556.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B)	-	1..72276	72276

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T138259 WP_001372321.1 NZ_CP043289:27385-27510 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T138259 NZ_CP057483:4041387-4041665 [Citrobacter sp. RHB25-C09]
ATGAATAAGCTTACTCAATACGATCCCGCCGCTGCGCTGGTCGATGACGACGAAATTGCTGTATTTATGGCAGATGCCCT
GGAAACGGGTGATGCGGCTTACATAGCAAAAGCGCTCGGCGTGGTGGCTCGCGCAAAAGGCATGGCAAACATTGCGGCTG
AAACCGGCTTATCACGCGAACAGCTTTATCGTTCATTCAGTGACAAAGGCAACCCCACGCTTAAAACCACGCTGGCGGTG
ATGAAGGCGCTGGGTATTGGATTAACTGTTAAAACATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT138259 NZ_CP043289:27241-27306 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin