Detailed information of TA system TA138235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1159729..1160456
• Replicon: chromosome
• Accession: NZ_CP043284
• Organism: Escherichia coli strain WCHEC025985

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: FYK42_RS05695
• Protein ID: WP_000547564.1
• Coordinates: 1159729..1160040 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FYK42_RS05700
• Protein ID: WP_000126294.1
• Coordinates: 1160037..1160456 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK42_RS05665 (1154871)	1154871..1156580	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
FYK42_RS05670 (1156590)	1156590..1157132	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
FYK42_RS05675 (1157132)	1157132..1157899	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
FYK42_RS05680 (1157896)	1157896..1158306	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
FYK42_RS05685 (1158299)	1158299..1158769	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
FYK42_RS05690 (1158794)	1158794..1159555	+	762	WP_001026446.1	hypothetical protein	-
FYK42_RS05695 (1159729)	1159729..1160040	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
FYK42_RS05700 (1160037)	1160037..1160456	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
FYK42_RS05705 (1160570)	1160570..1161994	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
FYK42_RS05710 (1162003)	1162003..1163459	-	1457	Protein_1124	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FYK42_RS05715 (1163719)	1163719..1164729	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
FYK42_RS05720 (1164878)	1164878..1165405	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T138235 WP_000547564.1 NZ_CP043284:1159729-1160040 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T138235 NZ_CP057481:c729764-729555 [Escherichia fergusonii]
ATGCTGAACACATGTAGAGTGCCTCTTACTGACCGTAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT138235 WP_000126294.1 NZ_CP043284:1160037-1160456 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT138235 NZ_CP043284:1160037-1160456 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5