Detailed information of TA system TA138225

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 73944..74365
• Replicon: plasmid p1_025157
• Accession: NZ_CP043282
• Organism: Escherichia coli strain WCHEC025157

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: FYK41_RS24250
• Protein ID: WP_096937776.1
• Coordinates: 74240..74365 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	73944..74142 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK41_RS24210 (69640)	69640..71034	+	1395	WP_001037799.1	carbohydrate porin	-
FYK41_RS24215 (71280)	71280..71384	-	105	Protein_79	thiosulfate reductase cytochrome B subunit	-
FYK41_RS24220 (71441)	71441..72134	+	694	Protein_80	IS1-like element IS1A family transposase	-
FYK41_RS24225 (72261)	72261..72773	+	513	WP_223350513.1	potassium channel family protein	-
FYK41_RS24230 (72858)	72858..73109	+	252	Protein_82	iron-containing alcohol dehydrogenase	-
FYK41_RS24235 (73398)	73398..73715	-	318	Protein_83	IS110 family transposase	-
FYK41_RS24240 (73870)	73870..73975	+	106	Protein_84	plasmid SOS inhibition protein A	-
- (73944)	73944..74142	+	199	NuclAT_0	-	Antitoxin
- (73944)	73944..74142	+	199	NuclAT_0	-	Antitoxin
- (73944)	73944..74142	+	199	NuclAT_0	-	Antitoxin
- (73944)	73944..74142	+	199	NuclAT_0	-	Antitoxin
FYK41_RS24245 (74149)	74149..74298	+	150	Protein_85	DUF5431 family protein	-
FYK41_RS24250 (74240)	74240..74365	+	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
FYK41_RS24255 (74666)	74666..74963	-	298	Protein_87	hypothetical protein	-
FYK41_RS24260 (75033)	75033..75239	+	207	WP_000275854.1	hypothetical protein	-
FYK41_RS24265 (75264)	75264..75551	+	288	WP_000107242.1	hypothetical protein	-
FYK41_RS24270 (75670)	75670..76491	+	822	WP_001234465.1	DUF932 domain-containing protein	-
FYK41_RS24275 (76787)	76787..77359	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
FYK41_RS24280 (77729)	77729..78112	+	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
FYK41_RS24285 (78303)	78303..78950	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
FYK41_RS24290 (79086)	79086..79301	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA17 / aadA5 / qacE / sul1 / mph(A)	-	1..116435	116435

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T138225 WP_096937776.1 NZ_CP043282:74240-74365 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T138225 NZ_CP057463:3422733-3423023 [Escherichia fergusonii]
ATGAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGGGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACGGGATTTGCCGGACTGCTACGAAATTA
AGCTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT138225 NZ_CP043282:73944-74142 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin