Detailed information of TA system TA138219

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72583..72847
• Replicon: plasmid pCMY42_025157
• Accession: NZ_CP043280
• Organism: Escherichia coli strain WCHEC025157

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: FYK41_RS23490
• Protein ID: WP_001387489.1
• Coordinates: 72583..72735 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	72787..72847 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK41_RS23450 (67675)	67675..68208	-	534	WP_001221666.1	lipocalin family protein	-
FYK41_RS23455 (68302)	68302..69447	-	1146	WP_039023136.1	extended-spectrum class C beta-lactamase CMY-42	-
FYK41_RS23465 (70652)	70652..70750	+	99	Protein_83	ethanolamine utilization protein EutE	-
FYK41_RS23470 (70822)	70822..71031	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
FYK41_RS23475 (71423)	71423..71599	+	177	WP_001054897.1	hypothetical protein	-
FYK41_RS24700 (71664)	71664..71759	-	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
FYK41_RS23485 (72260)	72260..72511	+	252	WP_001291964.1	hypothetical protein	-
FYK41_RS23490 (72583)	72583..72735	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (72787)	72787..72847	+	61	NuclAT_0	-	Antitoxin
- (72787)	72787..72847	+	61	NuclAT_0	-	Antitoxin
- (72787)	72787..72847	+	61	NuclAT_0	-	Antitoxin
- (72787)	72787..72847	+	61	NuclAT_0	-	Antitoxin
FYK41_RS23495 (73056)	73056..73430	+	375	WP_223349261.1	hypothetical protein	-
FYK41_RS23500 (73583)	73583..74740	+	1158	Protein_90	IS1380-like element ISEcp1 family transposase	-
FYK41_RS23505 (74796)	74796..75493	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
FYK41_RS23510 (75504)	75504..75812	-	309	WP_224717264.1	transcription termination/antitermination NusG family protein	-
FYK41_RS23515 (76254)	76254..76541	-	288	WP_000074855.1	conjugal transfer protein TraA	-
FYK41_RS23520 (76579)	76579..76737	-	159	Protein_94	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	erm(B) / mph(A) / aac(3)-IId / blaCTX-M-14 / blaCMY-42	-	1..77649	77649
	-	inside	IScluster/Tn	blaCMY-42	-	68302..75071	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T138219 WP_001387489.1 NZ_CP043280:c72735-72583 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T138219 NZ_CP057463:1615120-1615338 [Escherichia fergusonii]
ATGTCCGATAAGCCTTTAACCAAAACTGATTATTTGATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAACGTGT
CATCGAAAAAAATAAATACGAACTCTCTGACAATGAACTGGCGGTATTTTATTCAGCAGCTGATCACCGTCTCGCTGAAT
TGACCATGAATAAACTTTACGACAAGATCCCCTCCTCCGTATGGAAATTCATTCGCTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT138219 NZ_CP043280:72787-72847 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin