Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3752639..3753180 Replicon chromosome
Accession NZ_CP043279
Organism Escherichia coli strain WCHEC025157

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag FYK41_RS17930 Protein ID WP_000615983.1
Coordinates 3752902..3753180 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag FYK41_RS17925 Protein ID WP_000729703.1
Coordinates 3752639..3752899 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK41_RS17905 (3748314) 3748314..3749099 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FYK41_RS17910 (3749071) 3749071..3750783 + 1713 Protein_3526 flagellar biosynthesis protein FlhA -
FYK41_RS17915 (3751007) 3751007..3751504 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FYK41_RS17920 (3751680) 3751680..3752429 - 750 WP_000056849.1 C40 family peptidase -
FYK41_RS17925 (3752639) 3752639..3752899 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FYK41_RS17930 (3752902) 3752902..3753180 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FYK41_RS17935 (3753336) 3753336..3754076 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
FYK41_RS17940 (3754047) 3754047..3754814 - 768 WP_000333380.1 class II glutamine amidotransferase -
FYK41_RS17945 (3755020) 3755020..3755598 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA / rhs/PAAR / vgrG/tssI 3694936..3768149 73213


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T138215 WP_000615983.1 NZ_CP043279:3752902-3753180 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T138215 NZ_CP057463:260084-260187 [Escherichia fergusonii]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT138215 WP_000729703.1 NZ_CP043279:3752639-3752899 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT138215 NZ_CP043279:3752639-3752899 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References