Detailed information of TA system TA138214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3746442..3747136
• Replicon: chromosome
• Accession: NZ_CP043279
• Organism: Escherichia coli strain WCHEC025157

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: FYK41_RS17890
• Protein ID: WP_001263489.1
• Coordinates: 3746442..3746840 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FYK41_RS17895
• Protein ID: WP_000554758.1
• Coordinates: 3746843..3747136 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3742030)	3742030..3742110	-	81	NuclAT_11	-	-
- (3742030)	3742030..3742110	-	81	NuclAT_11	-	-
- (3742030)	3742030..3742110	-	81	NuclAT_11	-	-
- (3742030)	3742030..3742110	-	81	NuclAT_11	-	-
FYK41_RS17865 (3742706)	3742706..3743164	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FYK41_RS17870 (3743425)	3743425..3744882	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
FYK41_RS17875 (3744939)	3744939..3745460	-	522	Protein_3519	peptide chain release factor H	-
FYK41_RS17880 (3745456)	3745456..3745662	-	207	Protein_3520	RtcB family protein	-
FYK41_RS17885 (3745980)	3745980..3746432	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
FYK41_RS17890 (3746442)	3746442..3746840	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FYK41_RS17895 (3746843)	3746843..3747136	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FYK41_RS17900 (3747188)	3747188..3748243	-	1056	WP_001226164.1	DNA polymerase IV	-
FYK41_RS17905 (3748314)	3748314..3749099	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
FYK41_RS17910 (3749071)	3749071..3750783	+	1713	Protein_3526	flagellar biosynthesis protein FlhA	-
FYK41_RS17915 (3751007)	3751007..3751504	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA / rhs/PAAR / vgrG/tssI	3694936..3768149	73213

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T138214 WP_001263489.1 NZ_CP043279:c3746840-3746442 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T138214 NZ_CP057460:c167613-167302 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT138214 WP_000554758.1 NZ_CP043279:c3747136-3746843 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT138214 NZ_CP043279:c3747136-3746843 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3