Detailed information of TA system TA138195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 66926..67637
• Replicon: plasmid p2_035148
• Accession: NZ_CP043277
• Organism: Escherichia coli strain WCHEC035148

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: FYK53_RS24100
• Protein ID: WP_000162415.1
• Coordinates: 67335..67637 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FYK53_RS24095
• Protein ID: WP_000806445.1
• Coordinates: 66926..67264 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK53_RS24060	62083..62475	-	393	WP_000824759.1	hypothetical protein	-
FYK53_RS24065 (FYK53_24295)	62496..63104	-	609	WP_000776434.1	hypothetical protein	-
FYK53_RS24070 (FYK53_24300)	63388..64041	+	654	WP_001557715.1	hypothetical protein	-
FYK53_RS24075 (FYK53_24305)	64370..64699	+	330	WP_112031932.1	baseplate protein	-
FYK53_RS24080 (FYK53_24310)	64692..65885	+	1194	WP_074470350.1	phage tail protein	-
FYK53_RS24085 (FYK53_24315)	65919..66647	-	729	WP_109157909.1	hypothetical protein	-
FYK53_RS24090 (FYK53_24320)	66684..66869	-	186	Protein_75	hypothetical protein	-
FYK53_RS24095 (FYK53_24325)	66926..67264	-	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
FYK53_RS24100 (FYK53_24330)	67335..67637	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FYK53_RS24105 (FYK53_24335)	67800..68591	+	792	WP_112031933.1	phage tail protein	-
FYK53_RS24110 (FYK53_24340)	68588..69355	+	768	WP_000203293.1	hypothetical protein	-
FYK53_RS24115 (FYK53_24345)	69359..70339	+	981	WP_000046500.1	hypothetical protein	-
FYK53_RS24120 (FYK53_24350)	70336..70989	+	654	WP_087503501.1	baseplate protein	-
FYK53_RS24125 (FYK53_24355)	71049..71954	+	906	WP_112031934.1	recombination-associated protein RdgC	-
FYK53_RS24130 (FYK53_24360)	71998..72618	+	621	WP_044862276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89248	89248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T138195 WP_000162415.1 NZ_CP043277:c67637-67335 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T138195 NZ_CP057458:c2614433-2614179 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACTCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT138195 WP_000806445.1 NZ_CP043277:c67264-66926 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT138195 NZ_CP043277:c67264-66926 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAACAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3