Detailed information of TA system TA138188

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81977..82231
• Replicon: plasmid p1_035148
• Accession: NZ_CP043276
• Organism: Escherichia coli strain WCHEC035148

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: FYK53_RS23460
• Protein ID: WP_001312851.1
• Coordinates: 81977..82126 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	82170..82231 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK53_RS23430 (77224)	77224..78135	-	912	WP_000440183.1	carbamate kinase	-
FYK53_RS23435 (78146)	78146..79366	-	1221	WP_000410951.1	arginine deiminase	-
FYK53_RS23440 (80073)	80073..80687	+	615	Protein_86	VENN motif pre-toxin domain-containing protein	-
FYK53_RS23445 (80687)	80687..81133	-	447	Protein_87	plasmid replication initiator RepA	-
FYK53_RS23450 (81126)	81126..81200	-	75	WP_032336874.1	RepA leader peptide Tap	-
FYK53_RS23455 (81436)	81436..81693	-	258	WP_000083833.1	replication regulatory protein RepA	-
FYK53_RS23460 (81977)	81977..82126	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (82170)	82170..82231	+	62	NuclAT_0	-	Antitoxin
- (82170)	82170..82231	+	62	NuclAT_0	-	Antitoxin
- (82170)	82170..82231	+	62	NuclAT_0	-	Antitoxin
- (82170)	82170..82231	+	62	NuclAT_0	-	Antitoxin
FYK53_RS23465 (82370)	82370..82552	-	183	WP_000968309.1	hypothetical protein	-
FYK53_RS23470 (82653)	82653..83269	+	617	Protein_92	IS1-like element IS1A family transposase	-
FYK53_RS23475 (83307)	83307..84878	-	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
FYK53_RS23480 (84898)	84898..85245	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
FYK53_RS23485 (85245)	85245..85922	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
FYK53_RS23490 (85977)	85977..86066	+	90	Protein_96	IS1 family transposase	-
FYK53_RS23495 (86367)	86367..86579	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / qnrS1 / tet(A) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..122305	122305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T138188 WP_001312851.1 NZ_CP043276:c82126-81977 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T138188 NZ_CP057357:3645069-3645383 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT138188 NZ_CP043276:82170-82231 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin