Detailed information of TA system TA138169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1110102..1110829
• Replicon: chromosome
• Accession: NZ_CP043274
• Organism: Escherichia coli strain WCHEC035148

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: FYK53_RS05430
• Protein ID: WP_000547564.1
• Coordinates: 1110102..1110413 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FYK53_RS05435
• Protein ID: WP_000126294.1
• Coordinates: 1110410..1110829 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK53_RS05400 (1105244)	1105244..1106953	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
FYK53_RS05405 (1106963)	1106963..1107505	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
FYK53_RS05410 (1107505)	1107505..1108272	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
FYK53_RS05415 (1108269)	1108269..1108679	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
FYK53_RS05420 (1108672)	1108672..1109142	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
FYK53_RS05425 (1109167)	1109167..1109928	+	762	WP_001026446.1	hypothetical protein	-
FYK53_RS05430 (1110102)	1110102..1110413	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
FYK53_RS05435 (1110410)	1110410..1110829	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
FYK53_RS05440 (1110943)	1110943..1112367	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
FYK53_RS05445 (1112376)	1112376..1113833	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FYK53_RS05450 (1114093)	1114093..1115103	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
FYK53_RS05455 (1115252)	1115252..1115779	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T138169 WP_000547564.1 NZ_CP043274:1110102-1110413 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T138169 NZ_CP057353:3556124-3556342 [Citrobacter freundii]
ATGTCTGATAAACCATTAACTAAAACAGATTATTTGATGCGTCTTCGACGCTGCCAGACAATTGACACGCTCGAGCGCGT
TATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACGATGAATAAGCTGTACGACAAGATTCCAACTTCCGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT138169 WP_000126294.1 NZ_CP043274:1110410-1110829 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT138169 NZ_CP057353:3555722-3556096 [Citrobacter freundii]
ATGGACGAGTACTCACCCAAACGGCATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCTTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGCAACCATGGCTGGGTTAATGACCCGACTTCAGCAACCAGCCTGCAGCTCAACGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATTGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAATTATGGTATTAATACGCAGGATTTGCAGAAATGGCGAAAGTCCGGAAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5