Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 643891..644690 Replicon chromosome
Accession NZ_CP043274
Organism Escherichia coli strain WCHEC035148

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag FYK53_RS03150 Protein ID WP_000347251.1
Coordinates 643891..644355 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag FYK53_RS03155 Protein ID WP_001307405.1
Coordinates 644355..644690 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK53_RS03120 (638892) 638892..639326 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
FYK53_RS03125 (639344) 639344..640222 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
FYK53_RS03130 (640212) 640212..640991 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
FYK53_RS03135 (641002) 641002..641475 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
FYK53_RS03140 (641498) 641498..642778 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
FYK53_RS03145 (643027) 643027..643836 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FYK53_RS03150 (643891) 643891..644355 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FYK53_RS03155 (644355) 644355..644690 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FYK53_RS03160 (644839) 644839..646410 - 1572 WP_001273761.1 galactarate dehydratase -
FYK53_RS03165 (646785) 646785..648119 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
FYK53_RS03170 (648135) 648135..648905 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T138165 WP_000347251.1 NZ_CP043274:c644355-643891 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T138165 NZ_CP057353:799004-799411 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCACAGTGGATTTCGTTGCTCATTCATGGGCTAGTGGCGGT
GCTTATTTTATTGATGCCATGGCCACTGAGTTACACCCCTTTATGGTTGATTTTGCTCTCTCTGGTGGTGTTCGATTGCG
TGCGCAGTCAGCGCCGTATCAACGCTTGTCAGGGAGAGATTAGACTATTGATGGATGGCCGCCTGCGTTGGCAAGGGCAA
GAGTGGTCGATTGTTAGCGCACCCTGGATGATAAAAACGGGCATGATGTTGCGTTTGCGGTCAAGTACAGGAAAGCGTCA
GCATCTGTGGCTGGCGGCGGACAGCATGGATGATGCCGAATGGCGGGATTTGCGTCGGTTGATATTACAACCGTCAATGC
AGAAATAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT138165 WP_001307405.1 NZ_CP043274:c644690-644355 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT138165 NZ_CP057353:798757-799023 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGTCGCGGTATGCGTGAGCTGGACATTTCGATCATGCCGTT
TTTCGAACATGAGTATGACAACTTAAGTGATGACGAAAAGCGCATTTTTATCCGTTTGCTGGAATGTGATGACCCCGATT
TATTTAACTGGTTGATGAATCACGGGAAGCCGGCTGATGCACAGCTGGAGCAAATGGTACGGCTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References