Detailed information of TA system TA138125

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28827..29091
• Replicon: plasmid p3RM9974
• Accession: NZ_CP043268
• Organism: Escherichia albertii strain RM9974

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: FYK18_RS24690
• Protein ID: WP_001303307.1
• Coordinates: 28827..28979 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	29029..29091 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK18_RS24665 (23830)	23830..25998	+	2169	WP_000698368.1	DotA/TraY family protein	-
FYK18_RS24670 (26069)	26069..26731	+	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
FYK18_RS24675 (26803)	26803..27012	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
FYK18_RS24680 (27404)	27404..27580	+	177	WP_001054900.1	hypothetical protein	-
- (28066)	28066..28117	+	52	NuclAT_1	-	-
- (28066)	28066..28117	+	52	NuclAT_1	-	-
- (28066)	28066..28117	+	52	NuclAT_1	-	-
- (28066)	28066..28117	+	52	NuclAT_1	-	-
FYK18_RS24685 (28504)	28504..28755	+	252	WP_001291965.1	hypothetical protein	-
FYK18_RS24690 (28827)	28827..28979	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
- (29029)	29029..29091	+	63	NuclAT_0	-	Antitoxin
- (29029)	29029..29091	+	63	NuclAT_0	-	Antitoxin
- (29029)	29029..29091	+	63	NuclAT_0	-	Antitoxin
- (29029)	29029..29091	+	63	NuclAT_0	-	Antitoxin
FYK18_RS24695 (29271)	29271..30479	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
FYK18_RS24700 (30498)	30498..31568	+	1071	WP_000151575.1	IncI1-type conjugal transfer protein TrbB	-
FYK18_RS24705 (31561)	31561..33852	+	2292	WP_001289271.1	F-type conjugative transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..96650	96650

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T138125 WP_001303307.1 NZ_CP043268:c28979-28827 [Escherichia albertii]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T138125 NZ_CP057311:c1867775-1867368 [Escherichia fergusonii]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCATGGCCGTTAAGTTACACCCCGTTATGGATGGTGCTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACTTGTGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT138125 NZ_CP043268:29029-29091 [Escherichia albertii]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin