Detailed information of TA system TA138102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 257506..258200
• Replicon: chromosome
• Accession: NZ_CP043266
• Organism: Escherichia albertii strain RM9974

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: FYK18_RS01220
• Protein ID: WP_001263500.1
• Coordinates: 257802..258200 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FYK18_RS01215
• Protein ID: WP_000554758.1
• Coordinates: 257506..257799 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK18_RS01195 (253205)	253205..253702	+	498	WP_059275984.1	transposase	-
FYK18_RS01200 (253818)	253818..255530	-	1713	Protein_232	flagellar biosynthesis protein FlhA	-
FYK18_RS01205 (255502)	255502..256269	+	768	WP_072243690.1	putative lateral flagellar export/assembly protein LafU	-
FYK18_RS01210 (256399)	256399..257454	+	1056	WP_059217800.1	DNA polymerase IV	-
FYK18_RS01215 (257506)	257506..257799	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FYK18_RS01220 (257802)	257802..258200	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FYK18_RS01225 (258210)	258210..258662	+	453	WP_072243685.1	GNAT family N-acetyltransferase	-
FYK18_RS01230 (258675)	258675..259274	+	600	WP_265450798.1	peptide chain release factor H	-
FYK18_RS01235 (259290)	259290..259412	+	123	Protein_239	metal-dependent hydrolase	-
FYK18_RS01240 (259415)	259415..259657	-	243	WP_059222190.1	hypothetical protein	-
FYK18_RS01245 (259716)	259716..261173	-	1458	WP_001292996.1	cytosol nonspecific dipeptidase	-
FYK18_RS01250 (261434)	261434..261892	+	459	WP_001291989.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T138102 WP_001263500.1 NZ_CP043266:257802-258200 [Escherichia albertii]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T138102 NZ_CP057258:4307775-4308062 [Citrobacter freundii]
ATGAGTTATACGGTAAAGTTCAGGGAAGATGCGCTGAAAGAATGGCAAAAATTGGATAAAACCATTCAGCAGCAGTTTGC
CAAAGAACTTAAGAAATGCGGTGAAAATCCGCATGTCCCCTCCGCGAAGCTCAGGGGAATAAAAGATTGCTATAAAATCC
AACTACGCACTTCAAGGTTCCGTCTTGTTTATCAAGTTATTGACGACACCCTGGTCATCGCTGTCGTTGCCGTCGGTAAA
CGCGAGCGTAGTGAAGTTTATAATCTTGCCAGCGAAAGACTACGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT138102 WP_000554758.1 NZ_CP043266:257506-257799 [Escherichia albertii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT138102 NZ_CP057258:4307527-4307778 [Citrobacter freundii]
ATGCCACATATCATTTTAAGCGACACCACCGCCAGCGTGAGTGAGCTAAAAAAGAACCCGATGGCGACTGTTAGCGCAGG
GGATGGGTTTCCGGTCGCAATCCTGAACCGTAACCAGCCCGCCTTTTATTGTGTTCCCGCTGAGCTTTACGAAAAAATGC
TTGATGCGCTGGACGATCAGCAACTGGTTAAATTAGTCCATGAACGGAGCCAACAGCCACTGGTGGATGTTGATCTGGAC
AGCTATTTATGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3