Detailed information of TA system TA138029

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 61333..61587
• Replicon: plasmid pEc-050-NDM-5
• Accession: NZ_CP043230
• Organism: Escherichia coli strain Ec-050

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: FX998_RS25085
• Protein ID: WP_015387399.1
• Coordinates: 61381..61587 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	61333..61394 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FX998_RS25040	56985..57197	+	213	WP_005012601.1	hypothetical protein	-
FX998_RS25555	57498..57587	-	90	Protein_65	IS1 family transposase	-
FX998_RS25055	57642..58319	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
FX998_RS25060	58319..58666	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
FX998_RS25065	58686..60257	+	1572	WP_023149734.1	IS66 family transposase	-
FX998_RS25070	60295..60911	-	617	Protein_69	IS1 family transposase	-
FX998_RS25075	61012..61194	+	183	WP_000968309.1	hypothetical protein	-
-	61333..61394	-	62	NuclAT_0	-	Antitoxin
-	61333..61394	-	62	NuclAT_0	-	Antitoxin
-	61333..61394	-	62	NuclAT_0	-	Antitoxin
-	61333..61394	-	62	NuclAT_0	-	Antitoxin
FX998_RS25085	61381..61587	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
FX998_RS25090	61871..62128	+	258	WP_000083833.1	replication regulatory protein RepA	-
FX998_RS25100	62364..62438	+	75	WP_032336874.1	RepA leader peptide Tap	-
FX998_RS25105	62431..62877	+	447	Protein_74	incFII family plasmid replication initiator RepA	-
FX998_RS25110	62823..63305	-	483	Protein_75	VENN motif pre-toxin domain-containing protein	-
FX998_RS25115	64198..65418	+	1221	WP_000410951.1	arginine deiminase	-
FX998_RS25120	65429..66340	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IIa / mph(A) / sitABCD / tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5	iucA / iucB / iucC / iucD / iutA	1..99461	99461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T138029 WP_015387399.1 NZ_CP043230:61381-61587 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T138029 NZ_CP043230:61381-61587 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT138029 NZ_CP043230:c61394-61333 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin