Detailed information of TA system TA138023

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37363..37789
• Replicon: plasmid pEc-050-CMY-2
• Accession: NZ_CP043229
• Organism: Escherichia coli strain Ec-050

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FX998_RS24425
• Protein ID: WP_001312861.1
• Coordinates: 37363..37521 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37565..37789 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FX998_RS24375	32386..32613	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
FX998_RS24380	32750..33421	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
FX998_RS24385	33615..33998	-	384	WP_001354030.1	relaxosome protein TraM	-
FX998_RS24395	34321..34923	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
FX998_RS24400	35220..36041	-	822	WP_001234445.1	DUF945 domain-containing protein	-
FX998_RS24405	36152..36448	-	297	WP_001272251.1	hypothetical protein	-
FX998_RS24415	36674..36867	-	194	Protein_41	hypothetical protein	-
FX998_RS24425	37363..37521	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	37565..37789	-	225	NuclAT_0	-	Antitoxin
-	37565..37789	-	225	NuclAT_0	-	Antitoxin
-	37565..37789	-	225	NuclAT_0	-	Antitoxin
-	37565..37789	-	225	NuclAT_0	-	Antitoxin
FX998_RS25520	37601..37780	+	180	WP_001309233.1	hypothetical protein	-
FX998_RS24430	37801..38520	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
FX998_RS24435	38517..38951	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FX998_RS24440	39020..41043	-	2024	Protein_46	ParB/RepB/Spo0J family partition protein	-
FX998_RS24445	41104..41337	-	234	WP_000006003.1	DUF905 family protein	-
FX998_RS24450	41395..41922	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
FX998_RS24460	42150..42343	-	194	Protein_49	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2 / erm(B)	-	1..67616	67616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T138023 WP_001312861.1 NZ_CP043229:c37521-37363 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T138023 NZ_CP057229:c1053392-1053138 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT138023 NZ_CP043229:c37789-37565 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin