Detailed information of TA system TA138019

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 113627..113891
• Replicon: plasmid pEc-050-TEM-30
• Accession: NZ_CP043228
• Organism: Escherichia coli strain Ec-050

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: FX998_RS24190
• Protein ID: WP_001303307.1
• Coordinates: 113627..113779 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	113829..113891 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FX998_RS24165	109472..110134	+	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
FX998_RS24170	110232..110441	+	210	WP_032178830.1	hemolysin expression modulator Hha	-
FX998_RS24175	110773..111177	-	405	WP_001175009.1	IS200/IS605 family transposase	-
FX998_RS24180	111236..112462	+	1227	WP_024186316.1	transposase	-
FX998_RS25515	112507..112644	+	138	WP_047088497.1	hypothetical protein	-
FX998_RS24185	113304..113555	+	252	WP_001291965.1	hypothetical protein	-
FX998_RS24190	113627..113779	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	113829..113891	+	63	NuclAT_0	-	Antitoxin
-	113829..113891	+	63	NuclAT_0	-	Antitoxin
-	113829..113891	+	63	NuclAT_0	-	Antitoxin
-	113829..113891	+	63	NuclAT_0	-	Antitoxin
FX998_RS24195	114071..115279	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA1 / ant(3'')-Ia / qacE / sul1 / blaTEM-30 / floR / sul2	-	1..115935	115935

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T138019 WP_001303307.1 NZ_CP043228:c113779-113627 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T138019 NZ_CP057221:c131353-131048 [Escherichia fergusonii]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT138019 NZ_CP043228:113829-113891 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin