Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4016717..4017411 Replicon chromosome
Accession NZ_CP043227
Organism Escherichia coli strain Ec-050

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag FX998_RS19470 Protein ID WP_001263489.1
Coordinates 4017013..4017411 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FX998_RS19465 Protein ID WP_000554758.1
Coordinates 4016717..4017010 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FX998_RS19445 4012349..4012846 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FX998_RS19450 4013070..4014809 - 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
FX998_RS19455 4014754..4015539 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FX998_RS19460 4015610..4016665 + 1056 WP_001226164.1 DNA polymerase IV -
FX998_RS19465 4016717..4017010 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FX998_RS19470 4017013..4017411 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FX998_RS19475 4017421..4017873 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
FX998_RS19480 4018179..4018445 + 267 WP_001295202.1 hypothetical protein -
FX998_RS19485 4018357..4018914 + 558 WP_001352051.1 peptide chain release factor H -
FX998_RS19490 4018971..4020428 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
FX998_RS19495 4020689..4021147 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 4021743..4021823 + 81 NuclAT_12 - -
- 4021743..4021823 + 81 NuclAT_12 - -
- 4021743..4021823 + 81 NuclAT_12 - -
- 4021743..4021823 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T138016 WP_001263489.1 NZ_CP043227:4017013-4017411 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T138016 NZ_CP057221:c76861-76655 [Escherichia fergusonii]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT138016 WP_000554758.1 NZ_CP043227:4016717-4017010 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT138016 NZ_CP043227:4016717-4017010 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References