Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4010673..4011214 Replicon chromosome
Accession NZ_CP043227
Organism Escherichia coli strain Ec-050

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag FX998_RS19430 Protein ID WP_000615983.1
Coordinates 4010673..4010951 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag FX998_RS19435 Protein ID WP_000729703.1
Coordinates 4010954..4011214 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FX998_RS19415 4008255..4008833 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FX998_RS19420 4009039..4009806 + 768 WP_000333380.1 class II glutamine amidotransferase -
FX998_RS19425 4009777..4010517 - 741 WP_001225679.1 murein L,D-transpeptidase -
FX998_RS19430 4010673..4010951 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FX998_RS19435 4010954..4011214 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FX998_RS19440 4011424..4012173 + 750 WP_000056849.1 C40 family peptidase -
FX998_RS19445 4012349..4012846 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FX998_RS19450 4013070..4014809 - 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
FX998_RS19455 4014754..4015539 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T138015 WP_000615983.1 NZ_CP043227:c4010951-4010673 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T138015 NZ_CP057221:c73303-73022 [Escherichia fergusonii]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAAGTCGATAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTATTGCATACCGCACAAAAGTGGCCATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT138015 WP_000729703.1 NZ_CP043227:c4011214-4010954 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT138015 NZ_CP057221:c73569-73300 [Escherichia fergusonii]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCAGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References