Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1046689..1047191 Replicon chromosome
Accession NZ_CP043227
Organism Escherichia coli strain Ec-050

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FX998_RS05260 Protein ID WP_000767829.1
Coordinates 1046689..1046943 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FX998_RS05265 Protein ID WP_001259255.1
Coordinates 1046940..1047191 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FX998_RS05230 1041704..1041931 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
FX998_RS05235 1041945..1043003 - 1059 WP_087523589.1 transport protein YeeE -
FX998_RS05240 1043182..1044540 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FX998_RS05245 1044530..1044592 - 63 WP_010723108.1 membrane protein YoeI -
FX998_RS05250 1044807..1045736 - 930 WP_000803366.1 LysR family transcriptional regulator -
FX998_RS05255 1045782..1046606 - 825 WP_000754737.1 SDR family oxidoreductase -
FX998_RS05260 1046689..1046943 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FX998_RS05265 1046940..1047191 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FX998_RS05270 1047475..1047525 + 51 WP_001364200.1 his operon leader peptide -
FX998_RS05275 1047671..1048570 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FX998_RS05280 1048576..1049880 + 1305 WP_074514295.1 histidinol dehydrogenase -
FX998_RS05285 1049877..1050947 + 1071 WP_000108933.1 histidinol-phosphate transaminase -
FX998_RS05290 1050947..1052014 + 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T138005 WP_000767829.1 NZ_CP043227:c1046943-1046689 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T138005 NZ_CP057220:c679581-679372 [Escherichia fergusonii]
ATGCTGAACACATGTAGAGTGCCTCTTACTGACCGTAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATAAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGTGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT138005 WP_001259255.1 NZ_CP043227:c1047191-1046940 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT138005 NZ_CP057220:679573-679630 [Escherichia fergusonii]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References