Detailed information of TA system TA137968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1242978..1243705
• Replicon: chromosome
• Accession: NZ_CP043225
• Organism: Enterobacter hormaechei strain PG20180056

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FYA23_RS05920
• Protein ID: WP_047718997.1
• Coordinates: 1243394..1243705 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FYA23_RS05915
• Protein ID: WP_047718995.1
• Coordinates: 1242978..1243397 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA23_RS05900	1240052..1241173	-	1122	WP_015571201.1	glutamate--cysteine ligase	-
FYA23_RS05905	1241279..1242169	+	891	WP_017694363.1	oxidoreductase	-
FYA23_RS05910	1242220..1242654	-	435	WP_003858863.1	hypothetical protein	-
FYA23_RS05915	1242978..1243397	-	420	WP_047718995.1	helix-turn-helix domain-containing protein	Antitoxin
FYA23_RS05920	1243394..1243705	-	312	WP_047718997.1	type II toxin-antitoxin system HigB family toxin	Toxin
FYA23_RS05925	1244066..1245895	-	1830	WP_047718999.1	ATP-binding protein	-
FYA23_RS05930	1245892..1246935	-	1044	WP_148772190.1	SIR2 family protein	-
FYA23_RS05935	1247243..1247698	-	456	WP_047719002.1	hypothetical protein	-
FYA23_RS05940	1247725..1247832	-	108	WP_157843337.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-134)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12572.38 Da
• Isoelectric Point: 9.9435

>T137968 WP_047718997.1 NZ_CP043225:c1243705-1243394 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSMDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Nucleotide

• Length: 312 bp

>T137968 NZ_CP057210:c3660961-3660554 [Escherichia fergusonii]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACTTGTGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15407.49 Da
• Isoelectric Point: 4.7238

>AT137968 WP_047718995.1 NZ_CP043225:c1243397-1242978 [Enterobacter hormaechei]
MNYAAAIKAANALTNVLPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESSEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT137968 NZ_CP043225:c1243397-1242978 [Enterobacter hormaechei]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGTACTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCGGATAACCCGCTGGTAGAGATGCTTACTG
CCAAAATCGATAAGTACGAAAACGAGTCCTCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGTATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGCGACTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTGAACGGACAGCGCACGTTAACGCTGGATCATATGCGGGCGCTGGCGAAGCGTTTTGGTTTGCCGGTGA
GCGCATTTGTGGGGAATTGA