Detailed information of TA system TA137958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4076069..4076763
• Replicon: chromosome
• Accession: NZ_CP043217
• Organism: Escherichia coli O80:H26 strain EC-107

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: FYA02_RS20255
• Protein ID: WP_001263493.1
• Coordinates: 4076365..4076763 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FYA02_RS20250
• Protein ID: WP_000554757.1
• Coordinates: 4076069..4076362 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA02_RS20230	4071701..4072198	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
FYA02_RS20235	4072422..4074161	-	1740	WP_001325255.1	flagellar biosynthesis protein FlhA	-
FYA02_RS20240	4074106..4074891	+	786	WP_187424739.1	putative lateral flagellar export/assembly protein LafU	-
FYA02_RS20245	4074962..4076017	+	1056	WP_001226164.1	DNA polymerase IV	-
FYA02_RS20250	4076069..4076362	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FYA02_RS20255	4076365..4076763	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FYA02_RS20260	4076773..4077225	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
FYA02_RS20265	4077471..4077674	+	204	Protein_3878	RNA ligase RtcB family protein	-
FYA02_RS20270	4077673..4078194	+	522	Protein_3879	peptide chain release factor H	-
FYA02_RS20275	4078251..4079708	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
FYA02_RS20280	4079969..4080427	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FYA02_RS20285	4080519..4081763	+	1245	WP_000189541.1	esterase FrsA	-
-	4081023..4081103	+	81	NuclAT_9	-	-
-	4081023..4081103	+	81	NuclAT_9	-	-
-	4081023..4081103	+	81	NuclAT_9	-	-
-	4081023..4081103	+	81	NuclAT_9	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T137958 WP_001263493.1 NZ_CP043217:4076365-4076763 [Escherichia coli O80:H26]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T137958 NZ_CP057210:c578220-577756 [Escherichia fergusonii]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACTGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT137958 WP_000554757.1 NZ_CP043217:4076069-4076362 [Escherichia coli O80:H26]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT137958 NZ_CP057210:c578555-578220 [Escherichia fergusonii]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGGCTGGACAGCATTCATTACGAAATTCTACCTGGAGGGCAAGTATTTA
TGTGCCGATTGGGAGATGAACAGGAGGATCCTACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6