Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4070025..4070566 Replicon chromosome
Accession NZ_CP043217
Organism Escherichia coli O80:H26 strain EC-107

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag FYA02_RS20215 Protein ID WP_000615976.1
Coordinates 4070025..4070303 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FYA02_RS20220 Protein ID WP_000729704.1
Coordinates 4070306..4070566 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA02_RS20200 4067607..4068185 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FYA02_RS20205 4068391..4069158 + 768 WP_000333379.1 class II glutamine amidotransferase -
FYA02_RS20210 4069129..4069869 - 741 WP_001225679.1 murein L,D-transpeptidase -
FYA02_RS20215 4070025..4070303 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FYA02_RS20220 4070306..4070566 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FYA02_RS20225 4070752..4071525 + 774 WP_015675129.1 C40 family peptidase -
FYA02_RS20230 4071701..4072198 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FYA02_RS20235 4072422..4074161 - 1740 WP_001325255.1 flagellar biosynthesis protein FlhA -
FYA02_RS20240 4074106..4074891 + 786 WP_187424739.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T137957 WP_000615976.1 NZ_CP043217:c4070303-4070025 [Escherichia coli O80:H26]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T137957 NZ_CP057210:533746-534036 [Escherichia fergusonii]
ATGAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGGGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACGGGATTTGCCGGACTGCTACAAAATTA
AGCTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTGATCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT137957 WP_000729704.1 NZ_CP043217:c4070566-4070306 [Escherichia coli O80:H26]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT137957 NZ_CP057210:533514-533756 [Escherichia fergusonii]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAGGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References