Detailed information of TA system TA137950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 2295375..2296068
• Replicon: chromosome
• Accession: NZ_CP043217
• Organism: Escherichia coli O80:H26 strain EC-107

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: FYA02_RS11620
• Protein ID: WP_000415584.1
• Coordinates: 2295772..2296068 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: FYA02_RS11615
• Protein ID: WP_000650107.1
• Coordinates: 2295375..2295770 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA02_RS11605	2291239..2293497	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
FYA02_RS11610	2293635..2295242	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
FYA02_RS11615	2295375..2295770	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
FYA02_RS11620	2295772..2296068	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
FYA02_RS11625	2296273..2296755	-	483	WP_000183505.1	transcriptional regulator	-
FYA02_RS11630	2296808..2297200	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
FYA02_RS11635	2297352..2298011	+	660	WP_032205041.1	two-component system response regulator QseB	-
FYA02_RS11640	2298008..2299357	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
FYA02_RS11645	2299403..2299735	-	333	WP_000917684.1	DUF2645 family protein	-
FYA02_RS11650	2300054..2300635	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
FYA02_RS11655	2300666..2300980	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T137950 WP_000415584.1 NZ_CP043217:c2296068-2295772 [Escherichia coli O80:H26]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T137950 NZ_CP057150:2284672-2284950 [Citrobacter sp. RHB35-C17]
ATGATCATGAATTTCAGGCACAAGGGATTACGCGACCTGTTTCTTCACGGTAAAACGTCAGGCGTGCTGGCAAAGCACGT
CAAACGTTTGCGACACCGCCTCGCCGTGATTGACGCCGCAGGCTGCGTGAACGATATCAACATGCCCGGTTATCGGCTGC
ATCCTCTGAGCGGCGATCGTGATGGCGTCTGGTCAATATCGGTTTCAGACAACTGGCGTATGACGTTCGAATTTGTCAAT
GGCGATGCGTATATTCTGGATTACGAGGATTATCACTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT137950 WP_000650107.1 NZ_CP043217:c2295770-2295375 [Escherichia coli O80:H26]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT137950 NZ_CP057150:2284950-2285234 [Citrobacter sp. RHB35-C17]
ATGAAAATGGCGAATCATCCCCGCCCAGGGGATATCATTCAGGAAGCGCTGGACGAACTGAACGTCAGTTTGCGTGAATT
TGCCAGAGCAATGGAGATTGCGCCCTCAACCGCCAGCCGTTTACTCACGGGCAAAGCGGCACTCACCCCGGAAATGGCGA
TCAAACTCTCGGTGGTGATCGGCAGCTCCCCGGAAATGTGGCTAAATCTGCAAAATACCTGGAGTCTGGCACAAGCGCAA
AAAAGCGTGGACGTGTCCCGCCTGCGCCGTCTGGCGGTGCAGTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65