Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3701584..3702311 Replicon chromosome
Accession NZ_CP043213
Organism Escherichia coli O16:H48 strain CV601gfp

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag FYA04_RS18080 Protein ID WP_000550189.1
Coordinates 3701997..3702311 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag FYA04_RS18075 Protein ID WP_000560266.1
Coordinates 3701584..3702000 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA04_RS18065 3696744..3699095 + 2352 WP_000695487.1 alpha-glucosidase -
FYA04_RS18070 3699521..3701539 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
FYA04_RS18075 3701584..3702000 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
FYA04_RS18080 3701997..3702311 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
FYA04_RS18085 3702595..3703731 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
FYA04_RS18090 3703816..3704319 + 504 WP_001333820.1 M48 family metallopeptidase -
FYA04_RS18095 3704396..3705088 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
FYA04_RS18100 3705167..3706153 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T137929 WP_000550189.1 NZ_CP043213:c3702311-3701997 [Escherichia coli O16:H48]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T137929 NZ_CP057136:c4108365-4107967 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCACAGTGGATTTCGCTGCTCATTCATGGGCTAGTGGCGGT
GCTCATTTTATTGATGCCATGGCCACTGAGTTACACCCCATTATGGTTGATTTTGCTCTCTCTGGTGGTGTTCGATTGCG
TGCGCAGTCAGCGCCGTATTAACGCTTGTCAGGGAGAGATTAGACTATTAATGGATGGCCGCCTGCGTTGGCAAGGGCAA
GAGTGGACGATTGTTAGCGCACCCTGGATGATAAAAACGGGCATGATGTTGCGTTTGCGGTCAAGTACAGGAAAACGTCA
GCATCTGTGGCTGGCGGCGGACAGTATGGATGATGCCGAGTGGCGGGATTTGCGTCGGTTGATATTACAGCAGAAATAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT137929 WP_000560266.1 NZ_CP043213:c3702000-3701584 [Escherichia coli O16:H48]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT137929 NZ_CP043213:c3702000-3701584 [Escherichia coli O16:H48]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References