Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 710494..711188 Replicon chromosome
Accession NZ_CP043213
Organism Escherichia coli O16:H48 strain CV601gfp

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag FYA04_RS03315 Protein ID WP_001263489.1
Coordinates 710790..711188 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FYA04_RS03310 Protein ID WP_000554758.1
Coordinates 710494..710787 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA04_RS03290 706126..706623 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FYA04_RS03295 706847..708559 - 1713 Protein_645 flagellar biosynthesis protein FlhA -
FYA04_RS03300 708531..709316 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FYA04_RS03305 709387..710442 + 1056 WP_001226164.1 DNA polymerase IV -
FYA04_RS03310 710494..710787 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FYA04_RS03315 710790..711188 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FYA04_RS03320 711198..711650 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
FYA04_RS03325 711968..712174 + 207 Protein_651 RtcB family protein -
FYA04_RS03330 712170..712691 + 522 Protein_652 peptide chain release factor H -
FYA04_RS03335 712748..714205 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
FYA04_RS03340 714466..714924 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 715520..715600 + 81 NuclAT_12 - -
- 715520..715600 + 81 NuclAT_12 - -
- 715520..715600 + 81 NuclAT_12 - -
- 715520..715600 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T137898 WP_001263489.1 NZ_CP043213:710790-711188 [Escherichia coli O16:H48]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T137898 NZ_CP057104:c2146154-2145876 [Escherichia fergusonii]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGTGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATCTCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATCACATTTGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT137898 WP_000554758.1 NZ_CP043213:710494-710787 [Escherichia coli O16:H48]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT137898 NZ_CP057104:c2145876-2145592 [Escherichia fergusonii]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCGGCATTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTAGATGTGTCGCGACTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References