Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 704450..704991 Replicon chromosome
Accession NZ_CP043213
Organism Escherichia coli O16:H48 strain CV601gfp

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag FYA04_RS03275 Protein ID WP_000615983.1
Coordinates 704450..704728 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag FYA04_RS03280 Protein ID WP_000729703.1
Coordinates 704731..704991 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA04_RS03260 702032..702610 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FYA04_RS03265 702816..703583 + 768 WP_000333380.1 class II glutamine amidotransferase -
FYA04_RS03270 703554..704294 - 741 WP_001225679.1 murein L,D-transpeptidase -
FYA04_RS03275 704450..704728 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FYA04_RS03280 704731..704991 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FYA04_RS03285 705201..705950 + 750 WP_000056849.1 C40 family peptidase -
FYA04_RS03290 706126..706623 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FYA04_RS03295 706847..708559 - 1713 Protein_645 flagellar biosynthesis protein FlhA -
FYA04_RS03300 708531..709316 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T137897 WP_000615983.1 NZ_CP043213:c704728-704450 [Escherichia coli O16:H48]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T137897 NZ_CP057104:1889580-1889683 [Escherichia fergusonii]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT137897 WP_000729703.1 NZ_CP043213:c704991-704731 [Escherichia coli O16:H48]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT137897 NZ_CP057104:c1889721-1889453 [Escherichia fergusonii]
AAAGTCAGCGAATGGAATATTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References