Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3936519..3937060 Replicon chromosome
Accession NZ_CP043211
Organism Escherichia coli O16:H48 strain PG20180050

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag FYA05_RS19340 Protein ID WP_000615983.1
Coordinates 3936782..3937060 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag FYA05_RS19335 Protein ID WP_000729703.1
Coordinates 3936519..3936779 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA05_RS19315 3932194..3932979 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FYA05_RS19320 3932951..3934663 + 1713 Protein_3751 flagellar biosynthesis protein FlhA -
FYA05_RS19325 3934887..3935384 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FYA05_RS19330 3935560..3936309 - 750 WP_000056849.1 C40 family peptidase -
FYA05_RS19335 3936519..3936779 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FYA05_RS19340 3936782..3937060 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FYA05_RS19345 3937216..3937956 + 741 WP_001225679.1 murein L,D-transpeptidase -
FYA05_RS19350 3937927..3938694 - 768 WP_000333380.1 class II glutamine amidotransferase -
FYA05_RS19355 3938900..3939478 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T137882 WP_000615983.1 NZ_CP043211:3936782-3937060 [Escherichia coli O16:H48]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T137882 NZ_CP057101:993993-994328 [Escherichia fergusonii]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGTGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT137882 WP_000729703.1 NZ_CP043211:3936519-3936779 [Escherichia coli O16:H48]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT137882 NZ_CP057101:993745-993993 [Escherichia fergusonii]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References