Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2078165..2078667 Replicon chromosome
Accession NZ_CP043211
Organism Escherichia coli O16:H48 strain PG20180050

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FYA05_RS09835 Protein ID WP_000767829.1
Coordinates 2078413..2078667 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FYA05_RS09830 Protein ID WP_001259255.1
Coordinates 2078165..2078416 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA05_RS09805 2073343..2074410 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FYA05_RS09810 2074410..2075480 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
FYA05_RS09815 2075477..2076781 - 1305 WP_000009594.1 histidinol dehydrogenase -
FYA05_RS09820 2076787..2077686 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FYA05_RS09825 2077832..2077882 - 51 WP_001364200.1 his operon leader peptide -
FYA05_RS09830 2078165..2078416 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FYA05_RS09835 2078413..2078667 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FYA05_RS09840 2078750..2079574 + 825 WP_000754737.1 SDR family oxidoreductase -
FYA05_RS09845 2079620..2080549 + 930 WP_000803351.1 LysR family transcriptional regulator -
FYA05_RS09850 2080764..2080826 + 63 WP_010723108.1 membrane protein YoeI -
FYA05_RS09855 2080816..2082174 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FYA05_RS09860 2082353..2083411 + 1059 WP_000492339.1 transport protein YeeE -
FYA05_RS09865 2083425..2083652 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T137866 WP_000767829.1 NZ_CP043211:2078413-2078667 [Escherichia coli O16:H48]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T137866 NZ_CP043211:2078413-2078667 [Escherichia coli O16:H48]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT137866 WP_001259255.1 NZ_CP043211:2078165-2078416 [Escherichia coli O16:H48]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT137866 NZ_CP043211:2078165-2078416 [Escherichia coli O16:H48]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References