Detailed information of TA system TA137815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3645347..3646041
• Replicon: chromosome
• Accession: NZ_CP043207
• Organism: Escherichia coli O16:H48 strain PG20180053

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: FYA07_RS17965
• Protein ID: WP_001263489.1
• Coordinates: 3645347..3645745 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FYA07_RS17970
• Protein ID: WP_000554758.1
• Coordinates: 3645748..3646041 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3640935..3641015	-	81	NuclAT_12	-	-
-	3640935..3641015	-	81	NuclAT_12	-	-
-	3640935..3641015	-	81	NuclAT_12	-	-
-	3640935..3641015	-	81	NuclAT_12	-	-
FYA07_RS17940	3641611..3642069	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FYA07_RS17945	3642330..3643787	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
FYA07_RS17950	3643844..3644365	-	522	Protein_3497	peptide chain release factor H	-
FYA07_RS17955	3644361..3644567	-	207	Protein_3498	RtcB family protein	-
FYA07_RS17960	3644885..3645337	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
FYA07_RS17965	3645347..3645745	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FYA07_RS17970	3645748..3646041	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FYA07_RS17975	3646093..3647148	-	1056	WP_001226164.1	DNA polymerase IV	-
FYA07_RS17980	3647219..3648004	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
FYA07_RS17985	3647976..3649688	+	1713	Protein_3504	flagellar biosynthesis protein FlhA	-
FYA07_RS17990	3649912..3650409	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3632124..3646041	13917

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T137815 WP_001263489.1 NZ_CP043207:c3645745-3645347 [Escherichia coli O16:H48]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T137815 NZ_CP043207:c3645745-3645347 [Escherichia coli O16:H48]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT137815 WP_000554758.1 NZ_CP043207:c3646041-3645748 [Escherichia coli O16:H48]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT137815 NZ_CP043207:c3646041-3645748 [Escherichia coli O16:H48]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3