137782

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3645341..3646035 Replicon chromosome
Accession NZ_CP043205
Organism Escherichia coli O16:H48 strain PG20180054

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag FYA08_RS17970 Protein ID WP_001263489.1
Coordinates 3645341..3645739 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FYA08_RS17975 Protein ID WP_000554758.1
Coordinates 3645742..3646035 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3640929..3641009 - 81 NuclAT_12 - -
- 3640929..3641009 - 81 NuclAT_12 - -
- 3640929..3641009 - 81 NuclAT_12 - -
- 3640929..3641009 - 81 NuclAT_12 - -
FYA08_RS17945 3641605..3642063 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
FYA08_RS17950 3642324..3643781 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
FYA08_RS17955 3643838..3644359 - 522 Protein_3497 peptide chain release factor H -
FYA08_RS17960 3644355..3644561 - 207 Protein_3498 RtcB family protein -
FYA08_RS17965 3644879..3645331 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
FYA08_RS17970 3645341..3645739 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FYA08_RS17975 3645742..3646035 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FYA08_RS17980 3646087..3647142 - 1056 WP_001226164.1 DNA polymerase IV -
FYA08_RS17985 3647213..3647998 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FYA08_RS17990 3647970..3649682 + 1713 Protein_3504 flagellar biosynthesis protein FlhA -
FYA08_RS17995 3649906..3650403 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3632118..3664013 31895


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T137782 WP_001263489.1 NZ_CP043205:c3645739-3645341 [Escherichia coli O16:H48]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T137782 NZ_CP056967:1954161-1954264 [Citrobacter freundii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT137782 WP_000554758.1 NZ_CP043205:c3646035-3645742 [Escherichia coli O16:H48]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT137782 NZ_CP056967:c1954270-1954035 [Citrobacter freundii]
ACAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGTCCGCGACCAAAGTGG
CCTGAAAAAAAGGACGATTGTCACCCGCTAGAATGCAAAAACCGCAAGTTCTGCAGCCAGAACGTTGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References