Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3645166..3645860 Replicon chromosome
Accession NZ_CP043201
Organism Escherichia coli O16:H48 strain PG20180059

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag FYA10_RS17960 Protein ID WP_001263489.1
Coordinates 3645166..3645564 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FYA10_RS17965 Protein ID WP_000554758.1
Coordinates 3645567..3645860 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3640754..3640834 - 81 NuclAT_12 - -
- 3640754..3640834 - 81 NuclAT_12 - -
- 3640754..3640834 - 81 NuclAT_12 - -
- 3640754..3640834 - 81 NuclAT_12 - -
FYA10_RS17935 3641430..3641888 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
FYA10_RS17940 3642149..3643606 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
FYA10_RS17945 3643663..3644184 - 522 Protein_3497 peptide chain release factor H -
FYA10_RS17950 3644180..3644386 - 207 Protein_3498 RtcB family protein -
FYA10_RS17955 3644704..3645156 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
FYA10_RS17960 3645166..3645564 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FYA10_RS17965 3645567..3645860 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FYA10_RS17970 3645912..3646967 - 1056 WP_001226164.1 DNA polymerase IV -
FYA10_RS17975 3647038..3647823 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FYA10_RS17980 3647795..3649507 + 1713 Protein_3504 flagellar biosynthesis protein FlhA -
FYA10_RS17985 3649731..3650228 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T137716 WP_001263489.1 NZ_CP043201:c3645564-3645166 [Escherichia coli O16:H48]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T137716 NZ_CP056910:c2502051-2501773 [Citrobacter freundii]
ATGATCATGAACTTCAGACACAAAGGATTGCGCGACTTGTTTCTTCAGGGGCGAACATCAGGCGTGACGGCGAAGCATGT
CCGGCGATTGCGGCACCGACTTGCCGTTATTGATGCAGCGAGCCATGTGACAGATATCAATATGCCTGGTTATAAACTGC
ATGCGTTAACGGGCGATCGCGACGGCGTTTGGTCGATTTCTGTTCCAGGCAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGATGCATATATACTGGATTACGAGGACTATCATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT137716 WP_000554758.1 NZ_CP043201:c3645860-3645567 [Escherichia coli O16:H48]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT137716 NZ_CP056910:c2501773-2501489 [Citrobacter freundii]
ATGAAAATGGCTAATCATCCCCGTCCGGGGGATATTATTCAGGAAGCGCTGGATGAGTTAAACGTCAGCCTGCGCGAATT
TGCCAGAGCAATGGAGATTGCCCCCTCAACCGCGAGCCGACTGTTAACGGGCAAGGCAGCATTAACCCCGGAAATGGCGA
TCAAACTTTCAGTGGTGATCGGCAGTTCGCCCGAAATGTGGTTGAATCTGCAGAATGCCTGGAGTCTGGCTGAAGCGCAA
AAAAGCGTGGATATCTCACGTCTGCGCAAACTGGTAGCACAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References