Detailed information of TA system TA137687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 4210106..4210799
• Replicon: chromosome
• Accession: NZ_CP043199
• Organism: Escherichia coli O16:H48 strain PG20180060

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: FYA11_RS20490
• Protein ID: WP_000415584.1
• Coordinates: 4210503..4210799 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: FYA11_RS20485
• Protein ID: WP_000650107.1
• Coordinates: 4210106..4210501 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA11_RS20475	4205970..4208228	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
FYA11_RS20480	4208366..4209973	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
FYA11_RS20485	4210106..4210501	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
FYA11_RS20490	4210503..4210799	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
FYA11_RS20495	4211004..4211486	-	483	WP_000183505.1	transcriptional regulator	-
FYA11_RS20500	4211539..4211931	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
FYA11_RS20505	4212083..4212742	+	660	WP_001221495.1	two-component system response regulator QseB	-
FYA11_RS20510	4212739..4214088	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
FYA11_RS20515	4214134..4214466	-	333	WP_000917684.1	DUF2645 family protein	-
FYA11_RS20520	4214785..4215366	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
FYA11_RS20525	4215397..4215711	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T137687 WP_000415584.1 NZ_CP043199:c4210799-4210503 [Escherichia coli O16:H48]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T137687 NZ_CP056899:1033362-1033751 [Citrobacter sp. RHBSTW-00017]
ATGCAAACCAAATTATCCCCCCTCATGCGGGCGGCATCGTCACGCCCGTCTCCCGTGGGTGTCTGGCAGACCCTGCTGAC
CTGCCTGCTGGAACATCACTATGGTCTCACGCTTAACGATACGCCGTTCAGTGATGAACAGGTGATTCAGCAGCATATCG
ACGCAGGTATCTCGCTGTCCGATGCCCTGAATTTTATTGTAGAAAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATCACACCCATTGATATCCTCCGGGCCAGAAAGGCTGCGGGGCTGATGAACCGTGG
CACGTATAAAGAGGTGACGGCTATTACCAGAGGACAACATCCGCAGGCCAACGCTTCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT137687 WP_000650107.1 NZ_CP043199:c4210501-4210106 [Escherichia coli O16:H48]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT137687 NZ_CP043199:c4210501-4210106 [Escherichia coli O16:H48]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65