Detailed information of TA system TA137657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1271879..1272573
• Replicon: chromosome
• Accession: NZ_CP043199
• Organism: Escherichia coli O16:H48 strain PG20180060

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: FYA11_RS05925
• Protein ID: WP_001263489.1
• Coordinates: 1272175..1272573 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FYA11_RS05920
• Protein ID: WP_000554758.1
• Coordinates: 1271879..1272172 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA11_RS05900	1267511..1268008	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
FYA11_RS05905	1268232..1269944	-	1713	Protein_1137	flagellar biosynthesis protein FlhA	-
FYA11_RS05910	1269916..1270701	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
FYA11_RS05915	1270772..1271827	+	1056	WP_001226164.1	DNA polymerase IV	-
FYA11_RS05920	1271879..1272172	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FYA11_RS05925	1272175..1272573	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FYA11_RS05930	1272583..1273035	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
FYA11_RS05935	1273353..1273559	+	207	Protein_1143	RtcB family protein	-
FYA11_RS05940	1273555..1274076	+	522	Protein_1144	peptide chain release factor H	-
FYA11_RS05945	1274133..1275590	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
FYA11_RS05950	1275851..1276309	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	1276905..1276985	+	81	NuclAT_12	-	-
-	1276905..1276985	+	81	NuclAT_12	-	-
-	1276905..1276985	+	81	NuclAT_12	-	-
-	1276905..1276985	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T137657 WP_001263489.1 NZ_CP043199:1272175-1272573 [Escherichia coli O16:H48]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T137657 NZ_CP043199:1272175-1272573 [Escherichia coli O16:H48]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT137657 WP_000554758.1 NZ_CP043199:1271879-1272172 [Escherichia coli O16:H48]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT137657 NZ_CP043199:1271879-1272172 [Escherichia coli O16:H48]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3