Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3772938..3773440 Replicon chromosome
Accession NZ_CP043197
Organism Escherichia coli O16:H48 strain PG20180061

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FYA12_RS18155 Protein ID WP_000767829.1
Coordinates 3773186..3773440 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FYA12_RS18150 Protein ID WP_001259255.1
Coordinates 3772938..3773189 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA12_RS18125 3768116..3769183 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FYA12_RS18130 3769183..3770253 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
FYA12_RS18135 3770250..3771554 - 1305 WP_000009594.1 histidinol dehydrogenase -
FYA12_RS18140 3771560..3772459 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FYA12_RS18145 3772605..3772655 - 51 WP_001364200.1 his operon leader peptide -
FYA12_RS18150 3772938..3773189 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FYA12_RS18155 3773186..3773440 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FYA12_RS18160 3773523..3774347 + 825 WP_000754737.1 SDR family oxidoreductase -
FYA12_RS18165 3774393..3775322 + 930 WP_000803351.1 LysR family transcriptional regulator -
FYA12_RS18170 3775537..3775599 + 63 WP_010723108.1 membrane protein YoeI -
FYA12_RS18175 3775589..3776947 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FYA12_RS18180 3777126..3778184 + 1059 WP_000492339.1 transport protein YeeE -
FYA12_RS18185 3778198..3778425 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T137632 WP_000767829.1 NZ_CP043197:3773186-3773440 [Escherichia coli O16:H48]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T137632 NZ_CP056854:1888875-1888977 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT137632 WP_001259255.1 NZ_CP043197:3772938-3773189 [Escherichia coli O16:H48]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT137632 NZ_CP043197:3772938-3773189 [Escherichia coli O16:H48]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References