Detailed information of TA system TA137620

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 1282782..1283194
• Replicon: chromosome
• Accession: NZ_CP043197
• Organism: Escherichia coli O16:H48 strain PG20180061

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: FYA12_RS06375
• Protein ID: WP_000132601.1
• Coordinates: 1282853..1283194 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	1282782..1282858 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA12_RS06365	1279645..1281234	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
FYA12_RS06370	1281231..1282625	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
-	1282782..1282858	-	77	NuclAT_14	-	Antitoxin
-	1282782..1282858	-	77	NuclAT_14	-	Antitoxin
-	1282782..1282858	-	77	NuclAT_14	-	Antitoxin
-	1282782..1282858	-	77	NuclAT_14	-	Antitoxin
-	1282782..1282858	-	77	NuclAT_15	-	Antitoxin
-	1282782..1282858	-	77	NuclAT_15	-	Antitoxin
-	1282782..1282858	-	77	NuclAT_15	-	Antitoxin
-	1282782..1282858	-	77	NuclAT_15	-	Antitoxin
FYA12_RS06375	1282853..1283194	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
FYA12_RS06380	1283356..1284735	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
FYA12_RS06385	1284735..1285781	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T137620 WP_000132601.1 NZ_CP043197:1282853-1283194 [Escherichia coli O16:H48]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T137620 NZ_CP056852:977507-977830 [Citrobacter freundii]
ATGAAATTTCTACCTGCAACAAATATGCGGGCGGCGAAGCCATGCCTGTCGCCCGTGACTATCTGGCAGATGCTACTTAG
TCGTCTGCTGGAACAGCATTATGGCCTGACACTAAACGATACACCTTTCTGTGATGAAACCGTCATACAGGAACATATCG
ATGCCGGTATCACTCTCGCTAATGCCATTAACTTTCTAGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AGCTGGCAAGAACAAACGCCATATCTCACGATTATTGATATCATGAGGGCCCGTCGCGATCTGGGTTTAATGAATCGTAA
TTAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT137620 NZ_CP043197:c1282858-1282782 [Escherichia coli O16:H48]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin