Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 968342..969036 Replicon chromosome
Accession NZ_CP043197
Organism Escherichia coli O16:H48 strain PG20180061

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag FYA12_RS04915 Protein ID WP_001263489.1
Coordinates 968342..968740 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FYA12_RS04920 Protein ID WP_000554758.1
Coordinates 968743..969036 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 963930..964010 - 81 NuclAT_12 - -
- 963930..964010 - 81 NuclAT_12 - -
- 963930..964010 - 81 NuclAT_12 - -
- 963930..964010 - 81 NuclAT_12 - -
FYA12_RS04890 964606..965064 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
FYA12_RS04895 965325..966782 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
FYA12_RS04900 966839..967360 - 522 Protein_949 peptide chain release factor H -
FYA12_RS04905 967356..967562 - 207 Protein_950 RtcB family protein -
FYA12_RS04910 967880..968332 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
FYA12_RS04915 968342..968740 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FYA12_RS04920 968743..969036 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FYA12_RS04925 969088..970143 - 1056 WP_001226164.1 DNA polymerase IV -
FYA12_RS04930 970214..970999 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FYA12_RS04935 970971..972683 + 1713 Protein_956 flagellar biosynthesis protein FlhA -
FYA12_RS04940 972907..973404 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T137618 WP_001263489.1 NZ_CP043197:c968740-968342 [Escherichia coli O16:H48]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T137618 NZ_CP043197:c968740-968342 [Escherichia coli O16:H48]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT137618 WP_000554758.1 NZ_CP043197:c969036-968743 [Escherichia coli O16:H48]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT137618 NZ_CP043197:c969036-968743 [Escherichia coli O16:H48]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References