Detailed information of TA system TA137607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3933409..3934103
• Replicon: chromosome
• Accession: NZ_CP043195
• Organism: Escherichia coli O16:H48 strain PG20180062

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: FYA13_RS19310
• Protein ID: WP_001263489.1
• Coordinates: 3933409..3933807 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FYA13_RS19315
• Protein ID: WP_000554758.1
• Coordinates: 3933810..3934103 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3928997..3929077	-	81	NuclAT_12	-	-
-	3928997..3929077	-	81	NuclAT_12	-	-
-	3928997..3929077	-	81	NuclAT_12	-	-
-	3928997..3929077	-	81	NuclAT_12	-	-
FYA13_RS19285	3929673..3930131	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FYA13_RS19290	3930392..3931849	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
FYA13_RS19295	3931906..3932427	-	522	Protein_3744	peptide chain release factor H	-
FYA13_RS19300	3932423..3932629	-	207	Protein_3745	RtcB family protein	-
FYA13_RS19305	3932947..3933399	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
FYA13_RS19310	3933409..3933807	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FYA13_RS19315	3933810..3934103	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FYA13_RS19320	3934155..3935210	-	1056	WP_001226164.1	DNA polymerase IV	-
FYA13_RS19325	3935281..3936066	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
FYA13_RS19330	3936038..3937750	+	1713	Protein_3751	flagellar biosynthesis protein FlhA	-
FYA13_RS19335	3937974..3938471	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T137607 WP_001263489.1 NZ_CP043195:c3933807-3933409 [Escherichia coli O16:H48]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T137607 NZ_CP043195:c3933807-3933409 [Escherichia coli O16:H48]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT137607 WP_000554758.1 NZ_CP043195:c3934103-3933810 [Escherichia coli O16:H48]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT137607 NZ_CP056850:c403824-403528 [Citrobacter freundii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTACGTATAGAGAAAGGTGAAGAAACACCTAA
ACCTGAACATGTACATACTTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCGGTTGGTGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAACCCACTGGAGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAACCCATTTATAATTAATCAATTAAAAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3