Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2081251..2081753 Replicon chromosome
Accession NZ_CP043195
Organism Escherichia coli O16:H48 strain PG20180062

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FYA13_RS09840 Protein ID WP_000767829.1
Coordinates 2081499..2081753 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FYA13_RS09835 Protein ID WP_001259255.1
Coordinates 2081251..2081502 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA13_RS09810 2076429..2077496 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FYA13_RS09815 2077496..2078566 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
FYA13_RS09820 2078563..2079867 - 1305 WP_000009594.1 histidinol dehydrogenase -
FYA13_RS09825 2079873..2080772 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FYA13_RS09830 2080918..2080968 - 51 WP_001364200.1 his operon leader peptide -
FYA13_RS09835 2081251..2081502 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FYA13_RS09840 2081499..2081753 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FYA13_RS09845 2081836..2082660 + 825 WP_000754737.1 SDR family oxidoreductase -
FYA13_RS09850 2082706..2083635 + 930 WP_000803351.1 LysR family transcriptional regulator -
FYA13_RS09855 2083850..2083912 + 63 WP_010723108.1 membrane protein YoeI -
FYA13_RS09860 2083902..2085260 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FYA13_RS09865 2085439..2086497 + 1059 WP_000492339.1 transport protein YeeE -
FYA13_RS09870 2086511..2086738 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T137592 WP_000767829.1 NZ_CP043195:2081499-2081753 [Escherichia coli O16:H48]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T137592 NZ_CP043195:2081499-2081753 [Escherichia coli O16:H48]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT137592 WP_001259255.1 NZ_CP043195:2081251-2081502 [Escherichia coli O16:H48]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT137592 NZ_CP043195:2081251-2081502 [Escherichia coli O16:H48]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References