Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3645350..3646044 Replicon chromosome
Accession NZ_CP043193
Organism Escherichia coli O16:H48 strain PG20180063

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag FYA14_RS17975 Protein ID WP_001263489.1
Coordinates 3645350..3645748 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FYA14_RS17980 Protein ID WP_000554758.1
Coordinates 3645751..3646044 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3640938..3641018 - 81 NuclAT_12 - -
- 3640938..3641018 - 81 NuclAT_12 - -
- 3640938..3641018 - 81 NuclAT_12 - -
- 3640938..3641018 - 81 NuclAT_12 - -
FYA14_RS17950 3641614..3642072 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
FYA14_RS17955 3642333..3643790 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
FYA14_RS17960 3643847..3644368 - 522 Protein_3497 peptide chain release factor H -
FYA14_RS17965 3644364..3644570 - 207 Protein_3498 RtcB family protein -
FYA14_RS17970 3644888..3645340 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
FYA14_RS17975 3645350..3645748 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FYA14_RS17980 3645751..3646044 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FYA14_RS17985 3646096..3647151 - 1056 WP_001226164.1 DNA polymerase IV -
FYA14_RS17990 3647222..3648007 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FYA14_RS17995 3647979..3649691 + 1713 Protein_3504 flagellar biosynthesis protein FlhA -
FYA14_RS18000 3649915..3650412 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T137576 WP_001263489.1 NZ_CP043193:c3645748-3645350 [Escherichia coli O16:H48]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T137576 NZ_CP056830:c5267449-5267066 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT137576 WP_000554758.1 NZ_CP043193:c3646044-3645751 [Escherichia coli O16:H48]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT137576 NZ_CP056830:c5267691-5267449 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References