Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 609863..610662 Replicon chromosome
Accession NZ_CP043193
Organism Escherichia coli O16:H48 strain PG20180063

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag FYA14_RS02980 Protein ID WP_000347273.1
Coordinates 609863..610327 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag FYA14_RS02985 Protein ID WP_001307405.1
Coordinates 610327..610662 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA14_RS02955 606039..606596 - 558 Protein_575 amidohydrolase family protein -
FYA14_RS02960 606592..606963 - 372 Protein_576 PTS sugar transporter subunit IIC -
FYA14_RS02965 606974..607447 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
FYA14_RS02970 607470..608750 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
FYA14_RS02975 608999..609808 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FYA14_RS02980 609863..610327 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FYA14_RS02985 610327..610662 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FYA14_RS02990 610811..612382 - 1572 WP_001273753.1 galactarate dehydratase -
FYA14_RS02995 612757..614091 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
FYA14_RS03000 614107..614877 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 598879..621537 22658


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T137554 WP_000347273.1 NZ_CP043193:c610327-609863 [Escherichia coli O16:H48]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T137554 NZ_CP043193:c610327-609863 [Escherichia coli O16:H48]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT137554 WP_001307405.1 NZ_CP043193:c610662-610327 [Escherichia coli O16:H48]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT137554 NZ_CP056809:35008-35307 [Citrobacter freundii]
ATGGCTACCCTTAAAGAATTGATGGCGAAACAAAGCCCGGAAAGCCAGGAGCGTATTACCGAAAAAGTAGAAATACTACG
TCAGGCGGTTGCGTTAAATATGTTAAGGGAAGAACTGAATCTCTCTCAGGCTGAACTTGCCTCGGCAATGGGGGTTAAGC
AGCCAACAATCGCAAAAATGGAGCAGGCAGATAACGATCCTCGTCTGTCGACGCTCAAGCGTTATGTCACGGCACTGGGT
GGTGAACTTAGCATTGATGTTAAGCTACCTACAGGTAAAAGAGTCGCGTTCTATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References