Detailed information of TA system TA137456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3624979..3625673
• Replicon: chromosome
• Accession: NZ_CP043185
• Organism: Escherichia coli O16:H48 strain PG20180171

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: FYA18_RS17430
• Protein ID: WP_001263489.1
• Coordinates: 3625275..3625673 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FYA18_RS17425
• Protein ID: WP_000554758.1
• Coordinates: 3624979..3625272 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA18_RS17405	3620611..3621108	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
FYA18_RS17410	3621332..3623044	-	1713	Protein_3382	flagellar biosynthesis protein FlhA	-
FYA18_RS17415	3623016..3623801	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
FYA18_RS17420	3623872..3624927	+	1056	WP_001226164.1	DNA polymerase IV	-
FYA18_RS17425	3624979..3625272	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FYA18_RS17430	3625275..3625673	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FYA18_RS17435	3625683..3626135	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
FYA18_RS17440	3626453..3626659	+	207	Protein_3388	RtcB family protein	-
FYA18_RS17445	3626655..3627176	+	522	Protein_3389	peptide chain release factor H	-
FYA18_RS17450	3627233..3628690	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
FYA18_RS17455	3628951..3629409	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	3630005..3630085	+	81	NuclAT_12	-	-
-	3630005..3630085	+	81	NuclAT_12	-	-
-	3630005..3630085	+	81	NuclAT_12	-	-
-	3630005..3630085	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3610309..3663024	52715

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T137456 WP_001263489.1 NZ_CP043185:3625275-3625673 [Escherichia coli O16:H48]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T137456 NZ_CP056747:83162-83416 [Enterobacter hormaechei]
GTGAAGATCACATGGTCTGAAGAGGCATGGGAAGACTACCTCTATTGGCAGGAGACCGATAAGCGCATGGTGAGGAAAAT
CAATGAGCTTATCAAAATCTCCTGTCGCACTCCGTTTGAGGGGATCGGCAAGCCAGAGCCATTAAAACATAACCTGGCTG
GCTTCTGGTCTCGCCGAATCACCTCTGAGCATCGCATGGTCTATGCCATCACAGAAGAAGCGATCCTCATAGCTGCGTGC
CGATATCACTACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT137456 WP_000554758.1 NZ_CP043185:3624979-3625272 [Escherichia coli O16:H48]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT137456 NZ_CP043185:3624979-3625272 [Escherichia coli O16:H48]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3