Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 820577..821079 Replicon chromosome
Accession NZ_CP043185
Organism Escherichia coli O16:H48 strain PG20180171

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FYA18_RS04200 Protein ID WP_000767829.1
Coordinates 820577..820831 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FYA18_RS04205 Protein ID WP_001259255.1
Coordinates 820828..821079 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA18_RS04170 815592..815819 - 228 WP_000234896.1 sulfurtransferase TusA -
FYA18_RS04175 815833..816891 - 1059 WP_000492339.1 transport protein YeeE -
FYA18_RS04180 817070..818428 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FYA18_RS04185 818418..818480 - 63 WP_010723108.1 membrane protein YoeI -
FYA18_RS04190 818695..819624 - 930 WP_000803351.1 LysR family transcriptional regulator -
FYA18_RS04195 819670..820494 - 825 WP_000754737.1 SDR family oxidoreductase -
FYA18_RS04200 820577..820831 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FYA18_RS04205 820828..821079 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FYA18_RS04210 821362..821412 + 51 WP_001364200.1 his operon leader peptide -
FYA18_RS04215 821558..822457 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FYA18_RS04220 822463..823767 + 1305 WP_000009594.1 histidinol dehydrogenase -
FYA18_RS04225 823764..824834 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
FYA18_RS04230 824834..825901 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T137438 WP_000767829.1 NZ_CP043185:c820831-820577 [Escherichia coli O16:H48]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T137438 NZ_CP043185:c820831-820577 [Escherichia coli O16:H48]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT137438 WP_001259255.1 NZ_CP043185:c821079-820828 [Escherichia coli O16:H48]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT137438 NZ_CP043185:c821079-820828 [Escherichia coli O16:H48]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References